引用本文:
【打印本页】   【下载PDF全文】   【View/Add Comment】  【Download reader】  【 Close 】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被：浏览 2192次   下载 2634次 本文二维码信息
码上扫一扫！
字体:加大+|默认|缩小-
应用硫酸苯酚法建立一种甘露糖醛酸C-5差向异构酶活性检测方法的研究
林育姿1, 宫倩红1, 张真庆1, 韩?? 峰1, 于文功1
中国海洋大学医药学院
摘要:
建立了一种简便快捷的甘露糖醛酸C-5差向异构酶活性的测定 方法。研究发现相同分子质量的聚甘露糖醛酸和聚古罗糖醛酸与苯酚硫酸试剂反应后显色程度不同,以此可以用来测定β-D-甘露糖醛酸/α-L-古罗糖醛酸 (M/G)值的变化,从而计算甘露糖醛酸C-5差向异构酶的活性。利用本方法测定了已知结构的褐藻胶片断的M/G比,其结果与传统的1H-NMR测定值基 本一致。
关键词:  硫酸苯酚法  甘露糖醛酸C-5差向异构酶  酶活检测  
DOI：
分类号:
基金项目:国家自然科学基金资助项目(30371683)
A new method for enzymatic assay of C-5-mannuronan epimerase
Abstract:
A simple and convenient method for the C-5-mannuronan epimeration assay was established in this study. The color intensity of poly-mannuronate was markedly different from that of poly-guluronate when they reacted with sulfate-phenol. So the activity of C-5-mannuronan epimerase can be determined using the sulfate-phenol method by comparing the absorption of the alginate substrate before and after the enzymatic reaction. The M/G ratio of alginate fragments with known structure was detected by Sulfate-phenol method, and the result was almost the same as the value by 1H-NMR.
Key words:  sulfate-phenol  C-5-mannuronan epimerase  enzymatic assay  
Copyright ©  Editorial Office for Marine Sciences Copyright©2008 All Rights Reserved
Supervised by: Chinese Academy of Sciences (CAS)   Sponsored by: Institute of Oceanology, CAS
Address: 7 Nanhai Road, Qingdao, China.  Postcode: 266071  Tel: 0532-82898755  E-mail: bjb@qdio.ac.cn
Technical support: Beijing E-Tiller Co.,Ltd.