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柄海鞘脂肪酸提取工艺的研究
王长海¹, 张秀妍¹, 姜爱莉¹
烟台大学海洋学院

摘要:

用乙酸乙酯从柄海鞘(Styela clava)干粉中提取多不饱和脂肪酸,通过正交实验对提取工艺进行研究,得其最佳提取条件为:温度50℃,提取时间240 min,料液比1:10,提取次数2次。按此工艺,得脂肪酸产率为5.04%,利用气相色谱进行分析,得其中多不饱和脂肪酸质量分数为 67.79%,EPA和DHA的质量分数之和为19.47%。

关键词: 柄海鞘(Styela clava) 提取多不饱和脂肪酸 EPA DHA

DOI：

分类号:

基金项目:国家863计划资助项目(2001AA620416)

Extraction of fatty acid from Styela clava

Abstract:

Polyunsaturated fatty acid(PUFA) is extracted from dry powder of Styela clava by organic solvent. Extraction process is studied through orthogonal experiment and the optimization conditions of it is listed as follows: S. clava has been extracted with ethyl acetate at 50℃ for 240min, w(weight of ascidian): v(volume of ethyl acetate)=1:10, dry powder of S. clava is extracted twice, and the yield is 5.04%. According to the analysis of GC, the content of PUFA in the product is 67.79%, and the sum total content of EPA and DHA is 19.47%.

Key words: Styela clava extraction polyunsaturated fatty acid EPA DHA

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