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低分子量壳聚糖及其衍生物的制备与溶菌酶对其降解的实验研究
王 征1, 刘万顺1, 韩宝芹1, 魏长征1
中国海洋大学海洋生命学院
摘要:
将大分子质量壳聚糖用壳聚糖酶法降解成分子质量为3ku左 右的低分子质量壳聚糖,再将其分别化学修饰成低分子质量的羧甲基壳聚糖和羧甲基甲壳素,并进行结构表征。在最适条件下,用溶菌酶分别对3种多糖进行降解实 验,并进行酶解前后多糖的还原糖浓度测定和HPLC分析,确定溶菌酶对不同结构多糖的水解能力。结果表明,溶菌酶对羧甲基甲壳素水解作用最快,羧甲基壳聚 糖次之,壳聚糖最慢。该研究为甲壳素衍生物在生物医用材料中的应用和降解速率调控提供了依据。
关键词:  壳聚糖  羧甲基壳聚糖  羧甲基甲壳素  溶菌酶  降解
DOI：
分类号:
基金项目:国家863计划资助项目(2001AA625050)
Study on preparation of low molecular weight chitosan/its derivatives and hydrolysis by lysozyme
Abstract:
Low molecular weight chitosan(Mn=3 ku) is prepared from high molecular weight by chitosanase, carboxymethylchitoan and carboxymethychitin being synthesized by low molecular weight chitosan. The three amyloses were characterized by infrared spectrum and hydrolysised by lysozyme on the optimal condition. Their hydrolytic abilities in lysozyme are ascertained by lysozyme on the optimal conditions, by determining concentration of reducing sugar and High Performance Liquid Chromatography. The data revealed the hydrolysis rate of carbox ymethy chitin was fastest and that of chitosan was slowest.
Key words:  chitosan  carboxymethylchitoan  carboxymethychitin  lysozyme  hydrolysis
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