| 摘要: |
| 利用分光光度计比色法测定了温度、pH值和Hg2+等9种金 属离子对体长24.2cm±0.7cm仿刺参(Apostichopusjaponicus)消化道中蛋白酶活性的影响。结果表明,蛋白酶的最适温度为 40℃,对热不稳定,最适pH为7.2。Hg2+, Mn2+, Ag+, Pb2+, Ba2+, Ca2+对蛋白酶的活性有抑制作用,抑制作用由强至弱依次为 Hg2+>Ag+>Mn2+>Pb2+>Ca2+>Ba2+, 其中Hg2+和Ag+与对照差异极显著(P﹤0.01),表 现出极强的抑制作用; Cu2+, Zn2+, Mg2+则对酶活有一定的促进作用,其促进作用大小依次为Mg2+>Zn2+>Cu2+, Mg2+ 与对照差异显著(P﹤0.05)。 |
| 关键词: 仿刺参(Apostichopusjaponicus) 蛋白酶 温度 pH值 金属离子 |
| DOI： |
| 分类号: |
| 基金项目:辽宁省自然科学基金项目(20022109) |
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| Effects of temperature, pH value and metal ions on protease activities in the digestive tract from sea cucumber (Apostichopusjaponicus) |
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| Abstract: |
| Effects of temperature, pH and metal ions on protease activities in the digestive tract were studied in sea cucumber (Apostichopusjaponicus) with body length of 24.2 cm±0.7 cm by means of spectrophotometer. The results showed that the optimum temperature and pH value are 40℃ and 7.20, respectively. The metal ions including Hg2+, Mn2+, Ag+, Pb2+ , Ba2+, and Ca2+ were found to inhibit the protease activities while the Cu2+, Zn2+, and Mg2+ promote the activities. |
| Key words: sea cucumber (Apostichopusjaponicus) protease temperature pH value metal ions |
