| 摘要: |
| 检测了受三丁基锡(tributyltin,TBT)污染 的牡蛎的吞噬细胞活力、超氧化物歧化酶活性、过氧化氢酶活性、脂质过氧化作用等生理生化指标。实验结果表明,牡蛎血细胞的吞噬活力随着TBT浓度的增加而 下降;对不同TBT浓度下牡蛎的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性测定发现,在较低浓度下(TBT质量浓 度<10μg/L),SOD和CAT的酶活力被抑制,随着TBT浓度的增大,SOD和CAT的比活力也随着增大,但当TBT达到较高浓度时,SOD 和CAT的比活力开始下降,而过氧化脂质(LPO)却随着TBT的浓度的增加而增大。 |
| 关键词: 三丁基锡 僧帽牡蛎(Saccostrea cucullata) 过氧化氢酶 超氧化物歧化酶 过氧化脂质 |
| DOI： |
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| 基金项目:国家自然科学基金项目(30271033);广东省科技兴海重大项目(A200005F02) |
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| Effects of TBT on the phagocytic rate of blood cells and the activities of endogenous enzymes of protective systems in oyster (Saccostrea cucullata) |
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| Abstract: |
| Organotin compounds,especially tributyltin(TBT) and triphenyltin(TPHT), are widely used as biocidal additives in antifouling paints due to their effectiveness.However, they also cause damage to non-target organisms and are regarded as the most toxic materials ever deliberately introduced into the sea. In the paper,we study the effects of different TBT concentrations(0, 5, 10, 20, 30, 40 μg/L) on phagocytic rate of blood cells and the activities of endogenous enzymes of protective systems in oyster(Saccostrea cucullata). The endogenous enzymes of the protective systems included superoxide dismutase(SOD), catalase (CAT). The results showed that the phagocytic ability of blood cell in oyster decreased while the TBT concentration increased and the level of SOD and CAT activity were inhibited in the low concentration and enhanced gradually in the higher concentration, and sharply depressed in the highest concentration(30 μg/L to 40 μg/L). However,the lever of LPO was increased with TBT increase. |
| Key words: TBT Saccostrea cucullata superoxide dismutase (SOD) catalase(CAT),lipid peroxide(LPO) |
