| 摘要: |
| 于2004年4月,在浙江省宁波市镇海区澥浦滩涂采集野生大 弹涂鱼(Boleophthalmus pectinirostris)30尾,采用聚丙烯酰胺凝胶(PAGE)电泳方法,分析了乳酸脱氢酶(LDH)、甲酸脱氢酶(FDH)、谷氨酸脱氢酶 (GDH)、D-葡糖脱氢酶(GCDH)、葡萄糖-6-磷酸脱氢酶(G6PDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、超氧化物歧化酶 (SOD)、过氧化氢酶(CAT)和酯酶(EST)等10种酶在大弹涂鱼肝脏组织中的表达,并对同工酶表型进行了生化遗传分析。实验结果表明,大弹涂鱼 10种同工酶中除CAT外的另9种酶都得到了清晰的酶谱。该9种酶共记录了23个基因座位,筛选出LDH-1,FDH-1,G6PDH-1,ME- 1,SOD-1,EST-1,EST-2,EST-3等8个多态基因座位。澥浦大弹涂鱼多态座位比例(P)为34.78%,平均杂合度(H)为 0.0594,与其他海水鱼类相比,遗传多样性水平相对较低。 |
| 关键词: 大弹涂鱼(Boleophthalmus pectinirostris) 自然群体 同工酶 遗传多样性 |
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| 基金项目:浙江省财政厅和浙江省海洋与渔业局资助项目(503050005) |
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| Biochemical genetic analysis of isozymes in Boleophthalmus pectinirostris population in Xiepu |
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| Abstract: |
| In April 2004, 30 Boleophthalmus pectinirostris samples were collected from the lagune in Xiepu Villege, Zhenhai Town, Ningbo City, Zhejiang Province. Polyarylamide gel electrophoresis was used to detect the expression of isozymes in liver tissue. LDH, FDH, GDH, GCDH, G6PDH, MDH, ME, SOD, CAT and EST were analyzed and the biochemical genetic results showed that, among the 10 isozymes in this paper, 9 of which obtained clear enzyme patterns except for CAT. And 9 enzymes were coded by 23 genetic loci, 8 of which(LDH-1, FDH-1, G6PDH-1, SOD-1, EST-1, EST2, EST-3, ME-1)were found in polymorphica. The population of B. pectinirostris in Xiepu showed the lower genetic diversity compared with other seawater fishes, with 34.78% of the proportion of polymorphic loci and 0.0594 of the average heterozygosity per locus. |
| Key words: Boleophthalmus pectinirostris natural population isozymes genetic diversity |
