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三疣梭子蟹肌肉乳化病的同工酶病理变化研究
陈寅儿1, 王国良1, 金 珊1, 李 政1
宁波大学生命科学与生物工程学院
摘要:
采用垂直平板聚丙烯酰胺凝胶电泳技术（PAGE），对患有肌肉乳化病的三疣梭子蟹（Portunus trituberculatus）3 种组织中10 种同工酶及肌浆蛋白的酶谱表型进行分析,并就患病后酶谱变化特征及原因进行了讨论。证实在部分组织中，除SOD 酶外9 种酶及肌浆蛋白的酶带均出现明显的紊乱现象,表明病蟹的病理机制极可能与代谢异常有关,其特异性变化可以作为疾病早期诊断及病理分析的生化辅助指标。
关键词:  三疣梭子蟹（Portunus trituberculatus）  肌肉乳化病  同工酶
DOI：
分类号:
基金项目:浙江省科技厅项目(2003C32032); 浙江省自然科学基金项目(302440)
Analysis of the myogen and isozymes of the Portunus trituberculatus with emusification disease
Abstract:
The myogen (Pm),and 10 kinds of Isozymes in the 3 kinds of tissue of healthy Portunus trituberculatus and of individuais with emusification disease were studied by polyacrylamide gel electrophoresis. The result showed that the electrophoretogram changed greatly in the emusification disease Portunus trituberculatus. Compared with the isozyme patterns of some tissues from healthy crabs, those of diseased ones are different. It is considered that isozymes electrophoretogram can be used as a physiological as well as pathological index in the emusification disease.
Key words:  Portunus trituberculatus  emusification disease  isozymes
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