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海洋石油烃降解细菌T₁和T₂的分子鉴定分类
田胜艳^1,2, 王娟¹, 聂利红¹
1.天津科技大学海洋科学与工程学院;2.天津市海洋资源与化学重点实验室

摘要:

从天津滨海潮间带被石油烃严重污染的沉积物(千样含油量0.2g/g)中, 筛选分离出能够以柴油为唯一碳源生长的细菌, 对其中生物量大、单株菌降解效率较高的两株细菌T₁和T₂进行16S rDNA克隆, 通过测定和比较16S rDNA的部分序列对这两株细菌进行分子鉴定, 以期用于石油污染的微生物修复中。结果表明,T₁与深红红螺菌(Rhodospirillum rubrum)的同源性为89%,T₂与施氏甲单抱杆菌

关键词: 石油烃降解细菌菌 16S rDNA 分子鉴定甲单袍杆菌（Pseudomonas stutzeri）深红红螺菌（Rhodospirillum rubrum）

DOI：

分类号:

基金项目:天津科技大学科学研究基金资助项目(20060208)；天津市科技支撑计划重点项目(07CKFSF02000)

Molecular identification and classification of marine hydrocarbon degrading bacteria T₁ and T₂

Abstract:

Hydrocarbon degrading bacteria were obtained from oil-polluted sediments in the intertidal zone of Tianjin seashore by a soil enrichment procedure using diesel fuel as carbon source. The 16S rDAN amplified from 2 strains of these isolations, T₁ and T₂, which have a high biomass and a hydrocarbon degrading capability, was cloned. According to molecular criteria, nucleotides sequence of 16S rDAN was determined and analyzed. The results indicate that strain T₁ is similar to Rhodospirillum rubrum(89%) and strain T₂ is similar to Pseudomonas stutzeri(99%).

Key words: hydrocarbon degrading bacteria 16S rDNA molecular identification Pseudomonas stutzeri Rhodospirillum rubrum

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