| 摘要: |
| 为了了解红小丑鱼（Amphiprion frenatus）在人工饲养条件下繁殖和仔鱼培养的条件，采用实验生物学的方法，在严格控制的条件下培养亲鱼、胚胎和仔鱼。结果表明，红小丑每次产卵500～1 100粒，为黏性卵，成片集中附着在靠近海葵的坚硬物体表面，亲鱼有护卵行为。受精卵呈长椭圆形，长径为2.6 mm±0.2 mm，短径为0.9 mm±0.1 mm，经过9～10 d孵出仔鱼。初孵仔鱼平均体长3.84 mm±0.21 mm，对不饱和脂肪酸的依赖非常高，最好的饲喂方式是立即投喂经小球藻（Chlorella sp.）强化的褶皱臂尾轮虫（Brachionus plicatilis），密度在10个/mL左右，并保持到第7天。从第5天开始投喂经小球藻强化的卤虫(Artemia sp.)幼体。第6天进入稚鱼阶段，平均体长为6.32 mm±0.32 mm。到第18天，鱼苗已转入幼鱼阶段， 平均体长为14.83 mm±1.62 mm。幼鱼期投喂用小球藻或其他单胞藻饲养了2 d的小卤虫。25 d后，饵料从卤虫逐渐过渡到鱼糜或虾糜。 |
| 关键词: 红小丑（Amphiprion frenatus） 人工繁殖 育苗 |
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| Artifical breeding of the anemonefish Amphiprion frenatus |
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BAO Ying,ZHANG Peng,ZHU Cheng-yong,WANG Wei,LIU Ming
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| Abstract: |
| The anemonefish Amphiprion frenatus could ovulate under captive condition. The spawning interval was 13 to 15 days, all of the spawning started about 2 hours after sunrise in the morning, The spawning activity lasted about an hour, and about 100 to 1 100 eggs were produced each time. The adhesion demersal eggs, which were orange and ellipsoidal, were about 2.6 mm±0.2 mm long and 0.9 mm±0.1 mm in diameter. The eggs hatched after had been incubated approximately 10 days at 26℃ and the mean total length of newly hatched larvae was 3.84 mm±0.21 mm. Newly hatched larvae grew to 14.83 mm±1.62 mm (mean total length) in 18 days. In the first 4 days of larvae rearing, rotifers were kept at a constant density of 10 individuals/mL, and from the 5th day Artemia nauplii were supplied. The water was renewed about 20% everyday. The water temperature in the culture tank ranged from 24 to 26℃，pH ranged from 7.8 to 8.2，salinity ranged from 30 to 32，NH4-N was less than 0.1 mg/L，and dissolved oxygen content was more than 5.8 mg/L. |
| Key words: Amphiprion frenatus breeding larvae |
