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| L-精氨酸对凡纳滨对虾体液免疫因子的影响 |
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朱春华1,2, 李广丽1,2, 吴天力1,2, 师尚丽1,2, 吴灶和1,2
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1.广东海洋大学水产学院;2.广东省水产经济动物病原生物学和流行病学重点实验室
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| 摘要: |
| 用添加不同剂量L-精氨酸（L-arg）的饲料投喂凡纳滨对虾(Litopenaeus vannamei)，测定其血淋巴液中一氧化氮（NO）含量以及一氧化氮合成酶（NOS）、酸性磷酸酶（ACP）、碱性磷酸酶（AKP）和超氧化物歧化酶(SOD)活力，并检测其对病原体的抗病力。结果显示：L-arg显著提高血淋巴液中NO含量，且变化趋势与NOS、AKP活性升高趋势相一致；中等剂量（质量分数为1%）L-arg显著提高血淋巴液中SOD和ACP活性，但高剂量（质量分数为1.5%）、低剂量（质量分数为0.5%）L-arg对血淋巴液中SOD（高剂量除外）和ACP活性影响不显著。副溶血弧菌人工感染后，中剂量L-arg组（1%）对虾死亡率最低，高剂量（1.5%）、低剂量组（0.5%）次之，对照组死亡率最高，各剂量L-arg组死亡率与对照组相比差异显著。表明饲料中添加适量L-精氨酸可使对虾体液免疫因子总体活力达到较高水平，L-精氨酸含量过高对对虾非特异免疫力的作用效果反而不佳。 |
| 关键词: 凡纳滨对虾(Litopenaeus vannamei) L-精氨酸 一氧化氮合成酶 酸性磷酸酶 碱性磷酸酶 超氧化物歧化酶 |
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| 基金项目:广东省自然科学基金资助项目（5011783） |
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| Effects of L-arginine on the humoral immuning factors of Litopenaeus vannamei |
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ZHU Chun-hua,LI Guang-li,WU Tian-li,SHI Shang-li,WU Zao-he
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| Abstract: |
| The American white shrimp Litopenaeus vannam of the body length 6.8～8.5 cm was collected from a culture farm in Zhanjiang. One control group (raw diets) and three levels of L-arginine (L-arg) groups were designed. The levels of L-arginine added in the raw diets were 1.5%, 1% and 0.5%, respectively. The period was 4 weeks.Humoral immuning factors such as NOS, NO, ACP, AKP and SOD, as well as the sensitivity of the shrimp to Vibrio parahaemolyticus were investigated. The results showed that L-arginine at doses from 0.5% to 1.5% induced the increase of serum NO levels, which were in accordance with the increase of serum NOS and AKP levels. Both serum SOD and ACP levels increased significantly in the 1% L-arg-fed group, however, there was no significant difference in SOD(except for SOD in 1.5% L-arg-fed group) and ACP levels in 1.5% and 0.5% L-arg-fed groups. The lowest death rate was found in 1% L-arg-fed group, then in the 0.5% and 1.5% L-arginine-fed groups, and the highest death rate was found in the control group under infection V. parahaemolyticus. There was a significant difference in death rate between the control group and the L-arg-fed groups. The results suggested an adequate added level of L-arginine (e.g. 1%) could increase the activities of the immune factors in shrimp up to a high level, but the higher added level would not have a good effect on the non-specific immunity in shrimp. |
| Key words: Litopenaeus vannamei L-arginine NOS ACP AKP SOD |