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扁玉螺、罗氏海盘车中牛磺酸的HPLC测定
张晾1, 耿越1, 涂文利1, 张静静1
山东师范大学生命科学学院，山东省动物抗性生物学重点实验室
摘要:
利用高效液相色谱OPA柱前衍生法测定扁玉螺（Nevertia didyma (Roding)）肝脏、腹足，罗氏海盘车（Asterias rollestoni Bell）内脏、体壁中牛磺酸的含量。实验结果表明,100 g（湿质量）样品中牛磺酸含量分别为535.2、1 298.7、60.4、31.7 mg。
关键词:  牛磺酸  扁玉螺（Nevertia didyma (Roding)）  罗氏海盘车（Asterias rollestoni Bell）  高效液相色谱
DOI：
分类号:
基金项目:山东省优秀中青年科学家奖励基金项目(109034)
Determination of taurine in Nevertia didyma (Roding) and Asterias rollestoni Bell by HPLC
Abstract:
The contents of taurine in liver and abdominal foot of Nevertia didyma (Roding) and in viscera and body wall of Asterias rollestoni Bell were analyzed by high-performance liquid chromatography (HPLC). The analysis was based on the HPLC separation of the opthaldialdehyde derivative of taurine. The results showed that the taurine contents of liver and abdominal foot of N. didyma (Roding), viscera and body wall of A.rollestoni Bel1 were determined to be 535.2,1 298.7, 60.4 and 0.317 mg/g wet weight sample, respectively.
Key words:  taurine  Nevertia didyma (Roding)  Asterias rollestoni Bel1  HPLC
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