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4株形态不同的长心卡帕藻(Kappaphycus alvarezii)的初步ISSR分析
刘晨临1, 黄晓航1, 刘建国2
1.国家海洋局第一海洋研究所;2.中国科学院海洋研究所
摘要:
为了研究高产胶量品系的遗传学特征,建立了长心卡帕藻(Kappaphycus alvarezii)物种的ISSR分析方法。用ISSR技术对4株同一来源,形态各异的长心卡帕藻进行了遗传学分析,与11条ISSR引物共扩增得到60条带,其中1条带具有多态性并推测与稳定的经济性状连锁。说明ISSR技术可应用于长心卡帕藻品系筛选,结合其他手段,为分子标记辅助育种提供了可能。
关键词:  长心卡帕藻(Kappaphycus alvarezii)  ISSR  种质鉴定
DOI:
分类号:
基金项目:国家海洋公益项目(200705010)
The primary application of ISSR analysis on strains identification of Kappaphycus alvarezii
Abstract:
To investigate the genetic properties of high Carrangeenans production strains, the method of ISSR analysis of Kappaphycus alvarezii was established. The genetic diversities among 4 strains of K. alvarezii of the same origin which differs in configuration were analyzed. A total of 60 amplification loci were obtained from 11 ISSR primers, of which 1 locus revealed a polymorphism and possible linkage to the economic properties. It was indicated that the feasibility of ISSR could be combined with other measures on K. alvarezii.
Key words:  Kappaphycus alvarezii  inter-simple sequence repeat  strains of selection
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