| 摘要: |
| 作者采用酶学分析方法研究了反应介质中添加NaCl对三疣梭子蟹（Portunus tritubrculatus）中肠腺蛋白酶、胰蛋白酶、胰凝乳蛋白酶、羧肽酶A、羧肽酶B、淀粉酶活力的影响，反应介质中NaCl浓度设置为0、0.005、0.01、0.05、0.1、0.5、1.0和2.0 mol/L 8个梯度。实验结果表明，反应介质中添加NaCl对消化酶活力影响显著，除羧肽酶B外，添加NaCl对三疣梭子蟹消化酶均有激活作用，且所有消化酶都有很高的耐盐性。当反应介质中NaCl浓度为0.1～0.5 mol/L时蛋白酶活力最大；0.5 mol/L时胰蛋白酶活力最大；胰凝乳蛋白酶在反应介质中NaCl浓度为1 mol/L时酶活力最大；羧肽酶A在反应介质中NaCl 浓度为0.05～0.1 mol/L时酶活力最大；NaCl对羧肽酶B有抑制作用，在反应介质中NaCl浓度为2 mol/L的条件下，只有未添加NaCl时酶活力的60%；NaCl在0～2 mol/L时对淀粉酶均有激活作用，其最大激活能力在0.5 mol/L左右。
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| 关键词: 三疣梭子蟹（Portunus tritubrculatus） 消化酶 NaCl |
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| 基金项目:国家自然科学基金资助项目(30100140); 天津市农业科技成果转化与推广资助项目（07050201） |
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| The effects of NaCl on digestive enzyme activities of Portunus trituberculatus |
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HU Yi,PAN Lu-qing
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| Abstract: |
| Species composition and abundance of large tintinnids (>76 ?m) in the Yangtze River estuary were studied in four seasonal cruises in 2005. Samples were taken by vertically towing a Shallow Water III plankton net from bottom to surface. Totally 11 tintinnid species were found: Codonellopsis ostenfeldi，C. parva，Eutintinnus tenuis，Favella panamensis，Leprotintinnus nordqvisti，Leprotintinnus sp.，Tintinnopsis japonica，T. karajacensis，T. mortensenii，T. radix，and T. schotti. Maximum abundance of Leprotintinnus sp. was 32400 ind./m3 while maximum abundance of some species was lower than 200 ind./m3 (C. parva，E. tenuis and T. mortensenii)。And T. japonica mainly distributed in the area out of Yangtze River mouth in winter. Leprotintinnus sp. occurred in the area out of Yangtze River mouth in spring, summer and autumn. No tintinnid was found inside the Yangtze River in the four cruises.
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| Key words: Portunus trituberculatus metal NaCl digestive enzyme |
