| 摘要: |
| 采用5 mg /kg 17β-雌二醇对大菱鲆(Scophthalmus maximus)进行肌肉注射，一周后尾静脉取血，离心分离获得血浆,经Sephacryl S-300 (高分辨)分子筛纯化卵黄原蛋白，对卵黄原蛋白进行常规聚丙烯酰胺凝胶电泳，考马斯亮蓝染色和糖、磷、脂的特征性基团染色。结果表明，17β-雌二醇能诱导大菱鲆产生卵黄原蛋白，以Native-PAGE方法计算得到大菱鲆卵黄原蛋白的分子质量为538 ku，以SDS-PAGE方法得出其两种亚基的分子质量分别为100 ku和82 ku。分子筛凝胶过滤能较好地纯化大菱鲆血浆中的卵黄原蛋白。
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| 关键词: 大菱鲆（Scophthatmus maximus） 卵黄原蛋白 分离纯化 Native-PAGE SDS-PAGE |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目（30200211，30671618） |
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| Purification and partial characterization of turbot（Scophthatmus maximus） vitellogenin |
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YU Kun,RU Shao-guo,BING Xin,PAN Zong-bao,WANG Wei
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| Abstract: |
| Vitellogenin was induced in turbot (Scophthatmus maximus) by intramuscular injections of 17β-estradiol(5 mg/kg BW). After one week induction, the blood was collected from caudal vessel and then centrifuged. The blood plasma was loaded onto a Sephacryl S-300 high resolution column to purify the vitellogenin. Then the fraction was applied to Native-PAGE and the vitellogenin band was confirmed by the stainability with CBB G250-, phospho-, lipo- and glyco-staining methods. The result of Native-PAGE showed that the vitellogenin in turbot was significantly induced by treatment of 17β-estradiol for one week. Moreover, the vitellogenin showed a molecular weight of approximately 538 ku by Native-PAGE, and it was cleaved into two monomers in SDS-PAGE, with molecular weight of approximately 100 and 82 ku, respectively. Thus, the purification of vitellogenin in S. maximus was effectively established in this paper.
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| Key words: （Scophthatmus maximus） vitellogenin purification Native-PAGE SDS-PAGE |
