| 摘要: |
| 选择孔石莼(Uiva pertusa)和蜈蚣藻(Grateloupia filicina)两种海藻在挥发油测定器中用乙酸乙酯通过循环蒸馏萃取，浓缩后用电子轰击(electron impact， EI)源在全扫描检测模式下进行检测。各分离组分采用美国国家标准局NIST147及WILEY7谱库检索定性，采用色谱峰面积归一法进行相对定量。从两种海藻中共鉴定出61种半挥发性化合物，以醛类物质种类最多，半挥发性组分主要是醛类、脂肪酸类和醇类物质。两种海藻中含量最高的均是棕榈酸和十五醛。烘干对鉴定出的半挥发性物质种类没有影响，但对同一类物质，保留时间短的组分烘干后质量分数有增加的趋势，而保留时间长的组分质量分数有减少的趋势。该方法是一种灵敏、准确的分析方法，可以为海洋微藻中半挥发性成分的研究提供科学依据。 |
| 关键词: 海藻 半挥发性物质 气相色谱质谱联用 |
| DOI： |
| 分类号: |
| 基金项目:浙江省自然科学基金项目(Y506131); 教育部长江学者与创新团队项目 (IRT0734) |
|
| Analysis of the semivolatile organic compounds of two seaweeds |
|
WANG Xiu-juan,XU Ji-lin,YAN Xiao-jun
|
| Abstract: |
| The SVOCs in Uiva pertusa and Grateloupia filicina can be extracted by essential oil analyzer effectively， and determined using EI-SCAN-MS after concentration based on mass spectra and similarity retrieval from the two mass spectral databases (NIST98～147 and Wiley7 Mass Spectral Library). Sixty one SVOCs were identified and their relative contents were determined. It was found that the main SVOCs in the two seaweeds were aldehydes， fatty acids and alcohols. The high concentrations of SVOCs in these seaweeds were palmitic acid and pentadecanal.After being dried， the kinds of SVOCs were unchangeable and the SVOCs percentages with short retention time increased and those with long retention time decreased. A sensitive method for the determination of SOVCs in seaweeds was established and may provide a scientific basis for the study of SVOCs in seaweeds. |
| Key words: seaweed semivolatile organic compounds GC/MS |
