| 摘要: |
| 采用稀释涂板法研究了南麂岛海域沉积物中海洋放线菌的分离技术，分析了真空干燥处理、热处理、海水浓度、培养基种类等因素对分离效果的影响；首次将羧甲基纤维素钠(CMC)溶液应用于海洋沉积物样品的预处理，探讨了不同浓度的CMC对海洋沉积物样品中放线菌分离结果的影响。结果表明：海洋沉积物经干燥处理、50℃热处理20 min均能有效地减少细菌数量，利于海洋沉积物中放线菌的分离； 60%的海水配制的培养基对海洋放线菌的分离效果优于纯海水所配培养基，并且培养基中添加海泥浸出液能有效地增加海洋放线菌的出菌率；以质量浓度为2 g/L的CMC为分散剂时，不仅能良好地分散沉积物样品中的放线菌孢子，而且能明显地增加海洋放线菌的检出量及稀有放线菌的数量。 |
| 关键词: 南麂岛 海洋沉积物 海洋放线菌 分离方法 羧甲基纤维素钠(CMC) |
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| 基金项目:温州市科技计划资助项目(Y20080092) |
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| Isolation of marine actinomycetes strains from sediments in Nanji Island offshore |
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WANG Hai-yan,LIU Jian,ZHAO Shu-jiang
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| Abstract: |
| The isolation technologies of marine actinomycetes from the sediments in Nanji Island offshore were studied by using the gradient dilution and plate paint isolation methods. The influences of Vacuum drying, thermal treatment of the marine sediment samples, seawater concentration and medium variety on the isolation effect of marine actinomycetes strains were tested. And sodium carboxymethyl cellulose (CMC) was applied to the pretreatment of marine sediments for the first time. The results showed that bacteria decreased obviously in the samples by Vacuum drying and 50℃ thermal treatment for 20 minutes. The culture mediums made of 60% seawater were superior to those made of pure seawater. And adding mud leachate into the mediums could increase the strains of actinomycetes significantly. CMC of 2 g/L could disperse the spores of actinomycetes effectively, and increase the species of actinomycetes and their strains significantly. |
| Key words: Nanji Island marine sediment marine actinomycete isolation method sodium carboxymethyl cellulose (CMC) |
