| 摘要: |
| 建立了一种反相高效液相色谱法(RP-HPLC)测定浒苔(Enteromorpha prolifera)中叶绿素a和b含量。采用的色谱条件: Diamonsil-C18(4.6 mm×150 mm)柱, 流动相为甲醇(含0.2%的乙酸)和丙酮, 梯度洗脱, 流速为0.8 mL/min, 进样量10 mL, 检测波长430 nm。在此条件下, 叶绿素a和叶绿素b与浒苔中的其它化合物分离良好, 叶绿素a、b质量浓度为0.50～500 mg/L具有良好的线性关系(R2=0.999 9), 能对浒苔提取液中的叶绿素a、b进行准确定量。 |
| 关键词: 浒苔(Enteromorpha prolifera) 叶绿素 高效液相色谱法 |
| DOI： |
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| 基金项目:国家自然科学基金项目 (20905017, 20602009); 海洋公益性行业科研专项项目(200705011, 200805039); 国家海洋局 第一海洋研究所基本科研业务专项项目(GY-022008T32, GY-022008G47) |
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| Determination of chlorophyll a and chlorophyll b in Enteromorpha prolifera by ultrasound-assisted extraction with RP-HPLC |
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CHENG Hong-yan,CHEN Jun-hui,ZHANG Dao-lai,ZHAO Heng-qiang,ZHENG Li,ZANG Jia-ye,WANG Xiao-ru
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| Abstract: |
| A convenient and user-friendly reverse phase-high performance liquid chromatography (RP-HPLC) analysis method was established for the determination of chlorophyll a and chlorophyll b in Enteromorpha prolifera. The following HPLC conditions are follows: Diamonsil-C18 (4.6 mm×150 mm) column is used; MeOH-0.2% acetic acid and acetone as the mobile phase with linear gradient elution are used; flow-rate is 0.8 mL/min, detection length of Vis is 430 nm; injection volume is 10 μL. The results indicated that the developed method can be used for the determination of chlorophyll a and b in E. prolifera with a good resolution and linearity between 0.50～500 mg/L(R2=0.999 9).The method is specific, simple, fast, sensitive and feasible for the determination of chlorophyll a and chlorophyll b, which can eliminate the interference of chlorophyll derivatives in E. prolifera. |
| Key words: Enteromorpha prolifera Chlorophyll HPLC |
