| 摘要: |
| 为了确定鱼类神经坏死病毒对军曹鱼(Rachycentron canadum)仔鱼的致病性, 本研究通过肌肉注射、浸泡和共浴3 种途径分别感染军曹鱼仔鱼, 观察了由其导致的发病死亡情况和病理学变化。结果表明, 3 种感染途径都能造成军曹鱼仔鱼出现厌食、体色发黑和螺旋游泳等典型的病毒性神经坏死症症状, 累计死亡率分别达到78％、44％和34％。肌肉注射18 h 后, 利用RT-PCR 方法在鱼的脑、眼、脊髓中均可检测到该病毒。患病鱼的脑、视网膜、脊髓等组织发生空泡化, 与其他鱼类感染该病毒发生的变化一致。因此, 军曹鱼仔鱼是鱼类诺达病毒的易感种群之一, 病毒能通过水平传播感染军曹鱼仔鱼。
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| 关键词: 鱼类神经坏死病毒 军曹鱼(Rachycentron canadum)仔鱼 致病性 |
| DOI： |
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| 基金项目:广东省科技计划资助项目(2003B21502 , 2005B20301016) |
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| The research on pathogenicity of fish nervous necrosis virus to Rachycentron canadum juvenile |
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YAN Yun-feng,SU You-lu,GUO Zhi-xun,XU Li-wen,FENG Juan,HUANG Jian-nan,YU Da-hui
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| Abstract: |
| In this paper, the pathogenicity of fish nervous necrosis virus to Rachycentron canadum juveniles was studied by observations of the mortality and histo-pathological changes through three infected routes including intramuscular injection (IM), bath exposure and cohabitation. The results showed that the juveniles infected with NNV manifested the typical symptom such as severe sickness with anorexia, pale-grey pigmentation of body and whirling swimming. And the cumulative mortalities of infected fish via IM injection, bath exposure infection and cohabitation were 78%, 44% and 34%, respectively. NNV could be detected in the brain, eye and spinal cord of the infected R. canadum juveniles with RT-PCR at 18h post-IM injection. Microscopic observation revealed vacuolations in brain, retina and spinal cord of the infected juveniles. So the results demonstrated that R. canadum juveniles were susceptible to nervous necrosis virus and the virus could transmit horizontally among the juveniles by water.
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| Key words: fish nervous necrosis virus Rachycentron canadum juvenile pathogenicity |
