| 摘要: |
| 从海南省文昌县某养殖场患病方斑东风螺(Babylonia areolata)的吻、肌肉和肝脏中分离到2株优势菌。人工感染证实其中1 株为致病菌, 经形态学检测和生化鉴定, 该病原菌为鳗弧菌生物变种Ⅰ( Vibrio anguillarum BiovarⅠ)。该病原菌对方斑东风螺的LD50 值为1.7×106 cfu/g。药敏试验表明派拉西林、罗红霉素、红霉素、洁霉素、氟哌酸、先锋必、妥布霉素、菌必治、复方新诺明、利福平、复达欣、呋喃妥因等12 种抗生素类药物对该病原菌敏感。
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| 关键词: 方斑东风螺(Babylonia areolata) 鳗弧菌生物变种Ⅰ( Vibrio anguillarum BiovarⅠ) 分离 鉴定 药敏分析 |
| DOI： |
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| 基金项目:教育部新世纪优秀人才支持计划项目(NCET-05-0755); 科技部国际合作重点项目(2005DFA30610) |
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| Isolation, identification and antibiotic sensitivity analysis of bacterial pathogen from proboscis intumescence disease in Babylonia areolata |
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ZHANG Xin-zhong,WEN Wan-yao,FENG Yong-qin,XIE Zhen-yu,ZHOU Yong-can
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| Abstract: |
| Two dominant bacterial strains were isolated from the proboscis, muscle and liver of sick Babylonia areolata from a farm at Wengchang, Hainan. One of the stains was confirmed to be a bacterial pathogen of B. areolata by artificial infection and was determined to be Vibrio anguillarum BiovarⅠBased on morphological detection and biochemical tests. The LD50 of the pathogen to B. areolata was 1.7×106 cfu/g. Antibiotic sensitivity analysis indicated that the pathoge was sensitive to piperacillin, roxithromycin, cefobid, tobramycin, ceftriaxone, compound sulfamethoxazole,rifampicin, fortum and nitrofurantoin.
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| Key words: Babylonia areolata Vibrio anguillarum BiovarⅠ isolation identification antibiotic sensitivity analysis |
