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一株光合细菌菌株RPD-1的生物学特性及其在菲律宾蛤仔育苗中的应用
王 芳1, 李 筠2, 盛 军1, 孙 谧1
1.中国水产科学研究院黄海水产研究所;2.中国海洋大学
摘要:
RPD-1菌株分离自青岛栈桥近岸海水, 根据其生理生化特征鉴定为紫色非硫菌科红假单胞菌属(Rhodopseudomonas)。菌株RPD-1适盐范围较广, 在NaCl含量为0~8%的培养基中均可生长, 在NaCl为3%的培养基中生长最快; 在初始pH为6.0~9.0 的培养基中均可生长, pH为7.5时生长最快; 不同光源的光照条件下, 生长速度不同, 白炽灯光照下, 生长速度快, 日光灯照下, 生长速度慢; RPD-1菌株蛋白质含量为58.5%, 含各种必需氨基酸。菲律宾蛤仔(Ruditapes philippinarum)育苗实验结果表明,RPD-1菌株能提高菲律宾蛤仔幼虫的存活率和变态率; 水体氨态氮测定结果表明, RPD-1菌株能显著降低养殖水体中的氨氮。
关键词:  红假单胞菌属(Rhodopseudomonas)  菲律宾蛤仔(Ruditapes philippinarum)  存活率  变态率
DOI:
分类号:
基金项目:中央级科研院所基本科研项目(2007-gy-04)
Characterization of photosynthesis bacteria strain RPD-1 and its application in the breeding of Ruditapes philippinarum
WANG Fang,LI Jun,SHEN Jun,SUN Mi
Abstract:
The Photosynthesis Bacteria strain RPD-1 was isolated from seawater near the Qingdao Pier. According to physiological and biochemical characteristics, the RPD-1 strain was identified as Rhodopseudomonas sp. The RPD-1 strain growed well in the mediums containing 0~8% NaCl and the optimum salinity is 3%. Medium pH rangeing from 6.0 to 9.0 was appropriate for the RPD-1 strain, with the optimum pH being 7.5. The RPD-1 strain growed faster under the light of filament lamp than that of flurescent lamp. The protein content of RPD-1 strain was 58.5%. There are various necessary amino acids. The ratios of livability and metamorphosis of Ruditapes philippinarum were increased by adding the RPD-1 strain of suitable concentrations to the breeding water. When the RPD-1 strain of suitable concentration was added to the breeding water, the contents of nitrogen in the state of ammonia decreased significantly.
Key words:  Rhodopseudomonas  Ruditapes philippinarum  livability  metamorphosis
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