| 摘要: |
| 高温(26℃)培育刺参(Apostichopus japonicus)幼体获得选择群体, 生长至1.03 g±1.30 g, 与对照组幼参在18、20、22 和24℃温度下培育。二者的成活率随着温度升高而降低, 而选择组幼参24℃的成活率显著高于对照群体(P<0.05)。不同温度下的特定生长率之间没有差异。TRIzol 法提取刺参总RNA,获得刺参HSP70片断基因序列, 利用实时定量PCR方法检测,选择群体HSP70mRNA 的表达量在正常温度和热激条件下都较对照组高, 但表达量升高的倍数相近, 选择组为1.99 倍, 对照组为2.07 倍。 |
| 关键词: 刺参(Apostichopus japonicus) 特定生长率 选育 荧光实时定量PCR |
| DOI： |
| 分类号: |
| 基金项目:山东省农业良种工程项目 |
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| Effect of temperature on growth and HSP70 mRNA expression of thermo-tolerant brood of Apostichopus japonicus |
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LIU Guang-bin,YANG Hong-sheng,LIU Shi-lin
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| Abstract: |
| Under laboratory conditions, late auricularia of Apostichopus japonicus were reared at 26℃. Five days after the larvae completely metamorphosed, we transferred the juveniles, the so-called thermal-chosen brood, into sea water at natural temperature. This brood grew to 1.03 g±1.30 g. Comparative studies on the growth and different expression of HSP70 of this brood and the natural population were carried out. Data series like weight, density, and expression of HSP70 were recorded. Statistics showed that the survival rate of thermal-chosen group were significantly higher than the control group at 24℃(P<0.05). No significant differences of SGR and mRNA expressions of HSP70 were observed between the two groups. |
| Key words: Apostichopus japonicus (Selenka) SGR fluorescent real-time quantitative RT-PCR selected breeding |
