| 摘要: |
| 研究了两种大型海藻——羊栖菜(Sargassum fusiforme)、条浒苔(Enteromorpha clathrata)提取物对11 种水产动物病原菌的抑制活性。结果显示: 羊栖菜乙醇粗提物乙酸乙酯相对溶藻弧菌(Vibrio alginolyticus)的抑制作用最明显, 抑菌圈可达21.6 mm; 薄层层析表明羊栖菜乙醇粗提取物乙酸乙酯相有6条层析带, 其中Rf =0.54 带对哈氏弧菌(Vibrio harveyi)的抑制作用最强, 抑菌圈可达13.6 mm; 条浒苔乙醚粗脂对11 种水产动物病原菌均表现出了较强的抑制作用, 其中对灿烂弧菌(Vibrio splendidus)的抑制作用最强, 抑菌圈可达24.7 mm; 进一步的实验显示, 条浒苔乙醚粗脂3 种洗脱组分抗菌性均比粗脂减弱了, 而其95%乙醇洗脱组分对恶臭假单胞菌(Pseudomonas putida)的抑菌圈仍可达13.8 mm。说明利用羊栖菜、条浒苔为开发海洋药物资源提供了可能。 |
| 关键词: 羊栖菜(Sargassum fusiforme) 条浒苔(Enteromorpha clathrata) 提取物 水产动物病原菌 抗菌活性 |
| DOI： |
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| 基金项目:浙江省自然科学基金(Y506224); 温州市科技计划项目(S20100020) |
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| Antimicrobial activities of extracts from Sargassum fusiforme and Enteromorpha clathrata against aquatic animal pathogens |
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LU Yuan,YAN Mao-cang,XU Hong-xiang,NAN Chun-rong
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| Abstract: |
| Antimicrobial activities of extracts from two macro-algae, Sargassum fusiforme and Enteromorpha clathrata were assayed against 11 aquatic animal pathogens. The results showed that the ethyl acetate phase of ethanol extracts from S. fusiforme exhibited the strongest inhibition effect on Vibrio alginolyticus with an inhibition zone of 21.6 mm; the ethyl acetate phase of ethanol extracts from S. fusiforme had six chromatographic bands, among which the band with Rf =0.54 showed the strongest inhibition effect on Vibrio harveyi, with an inhibition zone was 13.6 mm; the crude fat of E. clathrata could inhibit all 11 aquatic animal pathogens, and the strongest inhibition effect was toward Vibrio splendidus with an inhibition zone of 24.7 mm. Moreover, the antimicrobial activities of the three elution fractions of the crude fat from E. clathrata was weaker than that of the crude fat, the inhibition zone of the 95% ethanol elution fractions to Pseudomonas putida was still up to 13.8 mm. Our results support the feasibility of developing marine drugs from S. fusiforme and E. clathrata. |
| Key words: Sargassum fusiforme Enteromorpha clathrata extract aquatic animal pathogen antimicrobial activity |
