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| 牙鲆dmrt1 基因的克隆及其与P450arom 基因的组织表达分析 |
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文爱韵1,2,3, 尤 锋1,2, 孙 鹏1,2, 徐冬冬1,2, 吴志昊1,2, 马得友1,2, 李 军1,2, 张培军1,2
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1.中国科学院海洋研究所;2.中国科学院实验海洋生物学重点实验室;3.中国科学院研究生院
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| 摘要: |
| 通过基因组步移和3’RACE 获得了牙鲆 (Paralichthys olivaceus) dmrt1 的cDNA 全序列, 该基因开放阅读框全长909bp, 其编码的蛋白具有高度保守的DM 结构域, 5’UTR 区含有性别相关转录因子Sox9 和Sox5 的结合位点。牙鲆dmrt1 基因只在牙鲆的性腺中表达, 且在精巢中的表达明显高于卵巢,表明牙鲆的dmrt1 可能是一种性别相关基因。同时, 对牙鲆的另一性别相关基因P450arom 在成体各组织的表达分析结果表明, 该基因除在牙鲆的性腺中有表达外, 在肾脏、脾脏、鳃和脑等其他组织中也有不同程度的表达。牙鲆P450arom 基因在性腺中的表达也存在两性差异, 其表达模式与dmrt1 的正好相反, 在卵巢中的表达量明显高于精巢。 |
| 关键词: 牙鲆(Paralichthys olivaceus) dmrt1 P450arom 两性差异表达 |
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| 基金项目:?国家973 项目(2010CB126304); 国家863 计划资助项目(2006AA10A404) |
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| Cloning of dmrt1 gene and its tissue expression analyses compared with that of P450arom gene in olive flounder (Paralichthys olivaceus) |
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| Abstract: |
| Whole cDNA sequence of olive flounder (Paralichthys olivaceus) dmrt1 gene was obtained using genome walking and 3’RACE methods. The open reading frame of flounder dmrt1 was found to span 909 bp. Sex-related transcriptional factors Sox9 and Sox5 binding sites were identified in its 5’UTR. Flounder Dmrt1 protein had a high conserved DM domain. Expressions of dmrt1 and P450arom genes in flounder tissues were also studied by RT-PCR. The results showed that both genes were sex-related genes. The dmrt1 gene was gonad- specific, and was sexual dimorphic with much higher expression in testis and much lower expression in ovary. The expression of P450arom in gonads was totally opposite to that of dmrt1. It was higher expressed in ovary and lower expressed in testis. Moreover, flounder P450arom had a broad-spectrum expression pattern, being differentially expressed in some other tissues, such as kidney, spleen, gill and brain. |
| Key words: Paralichthys olivaceus dmrt1 P450arom sexual dimorphic expression |