| 摘要: |
| ：通过实验生物学方法, 探讨了采用5℃条件保存的多细胞和两细胞状态羊栖菜(Hizikia fusiformis)受精卵的成活和发育情况。结果表明, 相同保存和恢复培养条件下的羊栖菜受精卵, 两细胞状态的死亡率明显高于多细胞状态。5℃条件保存60 h 多细胞状态羊栖菜受精卵的死亡率均在10%左右, 同样条件保存48 h 两细胞状态受精卵死亡率高达75%。在异地运输羊栖菜受精卵或者胚胎完成苗种培育的过程中, 应当同时考虑温度、运输时间以及受精卵的发育阶段等多个因素, 才能取得比较好的实际效果。 |
| 关键词: 羊栖菜(Hizikia fusiformis) 受精卵 低温保存 成活和发育 |
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| 基金项目:国家863计划项目 (2006AA10A412, 2006AA10A416); 国家自然科学基金项目(30671596, 30471327); 国家科技基础条件平台工作项目(2006DKA30470-017) |
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| Survival and growth performance of zygotes/embryos of the brown alga Hizikia fusiformis after low temperature treatments |
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| Abstract: |
| Hizikia fusiformis has been an important export-orientated brown alga in China. Large-scale cultivation relies on the supply of seedlings that are derived from sexual reproduction. With the development of the technique of synchronized fertilization, more and more seedlings are now produced via sexual reproduction. In this investigation, we performed a series of experiments to understand how zygotes of this alga develop and grow after short-term treatments at low temperature. Multi-celled embryos or two-cell zygotes of H. fusiformis were preserved at 5℃, for varied durations. The development of rhizoids, survivals of individuals, and growth performances were investigated. The results showed that the death rate of the two-cell zygote was conspicuously higher than that of the multi-cell zygote under the same conservation and recovering culture condition. The mortality of the multi-cell embryos was 10% after 60 h at 5℃, while that of the two-celled zygotes reached 75% after being preserved under the same condition. Discoveries in this investigation paved the way for transporting zygotes or embryos of this alga for completion of seedlings production over distance along the coast. |
| Key words: Hizikia fusiformis zygotes low temperature cultivation |
