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| 三种典型POPs 对紫贻贝不同组织DNA 损伤的比较研究 |
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周海龙1,2,3, 张林宝1,3, 曲 莹1,3, 邵立娜1, 刁晓平2, 郑继平2, 薛钦昭1
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1.中国科学烟台海岸带研究所;2.海南大学农学院;3.中国科学院研究生院
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| 摘要: |
| 采用彗星电泳技术, 比较研究了Aroclor 1254、BaP、DDT 三种典型POPs 对紫贻贝(Mytilus edulis)鳃、性腺、消化腺细胞的DNA 损伤效应的差异, 以期为进一步评价这三种典型POPs 遗传毒理机制及其预警监测提供科学依据。结果表明, Tail DNA%是评价DNA 损伤的理想指标, 另外, 在急性毒性试验中, 这三种POPs 对紫贻贝鳃、性腺、消化腺细胞的DNA 损伤作用均存在显著时间-剂量-效应关系,其中, BaP 的DNA 损伤作用最大, 鳃和消化腺细胞对毒性作用较敏感。同时, 对不同组织细胞的敏感性差异原因及其在环境监测中的应用进行了探讨。 |
| 关键词: POPs 紫贻贝(Mytilus edulis) 彗星电泳 DNA 损伤 |
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| 基金项目:中国科学院重大项目(KZCX2-YW-Q07-05, KZCX2-YWQ07-04) |
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| Comparative study of the DNA damage in three tissues of blue mussel (Mytilus edulis) after exposure to three typical POPs |
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| Abstract: |
| In this article, we compared the DNA damage in blue mussel gill, gonad and digestive gland cells that were exposed to Aroclor1254、BaP and DDT by comet assay. The results show that tail DNA% was a perfect parameter for DNA damage. In addition, there were significant time- and dose-dependent damage to the gill, gonad and digestive gland cells of blue mussel. Furthermore, the toxic effect of BaP was much more than the other two POPs; and gill and digestive gland cells were much more sensitive than gonad cells for the POPs toxicity. We also discussed the reason underlining the toxic sensitivity of different tissues and the significance of monitoring POPs in marine ecosystem. The results are helpful for assessing the toxic effects and exploring the toxicological mechanism of POPs. |
| Key words: POPs Blue mussel(Mytilus edulis) Comet assay DNA damage |