摘要: |
研究和评估了使用不同浓度MS-222 麻醉不同规格许氏平鲉(Sebastes schlegeli)幼鱼的效果。根据行为特征可将幼鱼的麻醉程度分为6 个时期, 复苏过程分为4 个时期。幼鱼达到IV 期麻醉之前呼吸频率略微增加, 达到IV 期麻醉时呼吸频率较明显下降。低浓度MS-222 对许氏平鲉幼鱼的呼吸频率影响较小, 在质量浓度20~80 mg/L 的MS-222 液中3 个规格的幼鱼呼吸频率较对照无显著差异; 幼鱼在高质量浓度MS-222 液(≥100 mg/L)中呼吸频率显著下降, 甚至停止呼吸进入休克状态。浓度越高, 幼鱼入麻时间越短, 复苏用时越长; 幼鱼个体规格越大, 需麻醉时间越长, 复苏用时也越长。MS-222 麻醉全长28.5~95.0 mm 许氏平鲉幼鱼的最佳质量浓度为80~100 mg/L。鱼体麻醉与死亡时刻间隔很短, 达到IV 期麻醉后应立即进行复苏, 否则会导致鱼体死亡。 |
关键词: 许氏平鲉(Sebastes schlegeli) MS-222 麻醉 |
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基金项目:海洋公益性行业科研专项(200805069); 山东省农业良种工程 |
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Anesthetic effect of MS-222 on juveniles of Sebastes schlegeli |
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Abstract: |
The anesthetic effect of MS-222 on Sebastes schlegeli juvenile was investigated. The anesthetic process of experimental fish can be divided into six stages and the recovery process four stages, according to their corresponding behaviors. The respiratory frequency of the fish was slightly increased when the fish were in the final anesthetic stage I~III, and then clearly declined at stage IV. MS-223 at low concentrations exerted slight influence on the respiratory frequency of the experimental fish. There was no remarkable difference in respiratory frequency between the 20~80 mg/L groups and the control group. The respiratory frequency of the sting fish was clearly declined, and even stopped in the high concentration MS-222 (≥100mg/L). The optimum concentrations of MS-222 on Sebastes schlegeli juvenile was 80mg/L. After up to 12 times of repeated anesthesia, the time needed for anesthesia and resuscitation of juvenile (TL=59.5mm) would be prolonged, indicating that anesthesia resistance capability can be improved by repeated anesthesia. In addition, the time margin between anesthesia and death was short. The fish should be removed for recovery immediately after being anesthetized to prevent death. |
Key words: Sebastes schlegeli MS-222 anesthetic |