| 摘要: |
| 从海参肠道中分离得到了一株海洋枯草芽孢杆菌(Bacillus subtilis)HS-A38, 将其发酵上清液经盐酸沉淀、有机溶剂萃取得到具有广谱抗菌活性的代谢物质。采用薄层层析和茚三酮显色检测到4 种物质, 分别命名为化合物1、2、3 和4; 应用层析板覆盖指示菌的生物自显影技术对其抗菌活性成分进行追踪、检测, 结果发现化合物1 和2 具有显著抑制弧菌的作用。进一步检测分析表明, 化合物1、2和4 具有很强的热稳定性, 初步推断它们属于直链的脂肽类化合物, 并且这4 种物质出现在不同的发酵代谢时期, 这将为研究群体效应提供基础检测依据。 |
| 关键词: 枯草芽孢杆菌(Bacillus subtilis) 抗菌活性 生物自显影 直链脂肽化合物 |
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| 基金项目:国家自然科学基金项目(31072224) , 辽宁省教育厅创新团队资助项目(LT2010012); 辽宁省自然科学基金项目(20102009) |
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| Antimicrobial activity of liner lipopeptides produced by Bacillus subtilis HS-A38 |
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| Abstract: |
| A marine Bacillus subtilis strain HS-A38 was isolated from the sea cucumber intestine. The crude extract of fermentation broth was prepared by methods of hydrochloric acid precipitation and solvent extraction. The extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. Four components, named as compound 1, 2, 3, and 4 were found by the thin layer chromatography and ninhydrin color printing. The compounds with antimicrobial activity were detected and tracked by bioautography of Vibrio parahaemolyticus and triphenyltetrazolium chloride (TTC) coloration on the plate of thin layer chromatography. Two inhibition spots were found, corresponding to compound 1 and 2. The further study showed that the compound 1, 2, and 4 were stable at high temperature. Therefore, it was preliminarily deduced that the four compounds were all straight-chain lipopeptide produced at different phase of fermentation. |
| Key words: Bacillus subtilis antimicrobial activity bioautography liner lipopeptides |
