| 摘要: |
| 以光镜和电镜观察了日本蟳(Charybdis japonica)血细胞的形态结构, 分析了各类血细胞的大小、数量比例及功能, 并测定了不同发育时期及饥饿状态的日本蟳血清主要生化指标。结果表明: 日本蟳血细胞可分为4 种类型, 从小到大依次为: 无颗粒细胞、小颗粒细胞、中间型细胞和大颗粒细胞; 其核质比分别为: 53.23%、33.64%、24.50%和19.45%; 数量比例分别为: 4.78%、42.69%、36.57%和15.96%。雌蟹血细胞密度显著高于雄蟹, 而细胞大小在不同性别之间差异不显著。吞噬能力从强到弱依次为中间型细胞、大颗粒细胞和小颗粒细胞, 无颗粒细胞无吞噬能力。鳃中血细胞表现出更为活跃的吞噬作用。生长期雌蟹血清中总蛋白、葡萄糖和总胆固醇含量均高于雄蟹, 成熟期雄蟹血清总蛋白含量高于雌蟹。雌蟹抱卵后, 血清中维持较高的总蛋白和总胆固醇水平。饥饿 7 d 的日本蟳血细胞密度及血清总蛋白、葡萄糖、总胆固醇含量明显下降, 谷丙转氨酶、碱性磷酸酶等酶活性显著降低, 而肌酐和尿素氮含量明显增加, 日本蟳血清中含有球蛋白。 |
| 关键词: 日本蟳(Charybdis japonica) 血细胞 血液生化指标 吞噬能力 饥饿 |
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| 基金项目:江苏省海洋生物技术重点建设实验室开放课题(Z2007HS006); 江苏省科技厅苏北科技发展计划项目(BN2010026) |
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| Hemocytes and blood biochemical characterization of Charybdis japonica |
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| Abstract: |
| In order to provide data for tissue pathology, disease control, and reproductive physiological research of Charybdis japonica, this paper reported the hemocytes and the blood biochemical characterization of Charybdis japonica. With light and electronic microscopes, four types of hemocytes, including agranular hemocyte (AG), small granular hemocyte (SG), intermediate granular hemocyte (IG) and large granular hemocyte (LG), were classified according to their granular existence, granular size, refractivity, staining characters, and nuclear-cytoplasmic ratio. The nuclear-cytoplasmic ratios were 53.23%(AG), 33.64%(SG), 24.50% (IG) and 19.45% (LG), respectively. Among these hemocytes, the percentages of AG, SG, IG and LG were 4.78%, 42.69%, 36.57%, and 15.96%, respectively. The female crab had higher density of hemocytes than the male, but there was no significant difference in cell size. The phagocytic capacity of hemocytes from strong to weak were IG, LG and SG. AG had no phagocytic capacity. The hemocytes in gill had big pseudopodia and abundant residual body suggesting they could be more active in phagorytosis. In the developing phase, the values of total protein(TP), glucose(GLU) and cholesterin(CHOL) of female were higher than those of male, but the male crabs in maturation had more TP than the female. After spawning, the female crabs could maintain high levels of TP and CHOL. After starvation for seven days, the values of density of hemocytes, TP, GLU, and CHOL decreased obviously and there were lower activities of alanine aminotransferase(ALT) and alkaline phosphatase(ALP) than before, but the levels of creatinine(CREA) and usea nitrogen(BUN) increased. Globin was found in the blood serum of Charybdis japonica. |
| Key words: Charybdis japonica hemocyte blood biochemical value phagocytic capacity starvation |
