| 摘要: |
| 首次在长牡蛎(Crassostrea gigas)中克隆得到细胞周期蛋白B3(cyclin B3)的cDNA 全长序列和基因组结构序列。cyclin B3 基因cDNA 全长2383 bp, 其中编码区长度为1293 bp, 编码一条含430 个氨基酸的多肽链, 氨基酸序列比对和结构域分析均表明其为其他物种cyclin B3 的同源蛋白, 预测的蛋白大小为47.8 kD。cyclin B3 基因含有10 个外显子和9 个内含子, 外显子和内含子的数目和大小与其物种的进化地位相符。Real Time PCR 分析表明, 该基因表达有较强的组织特异性, cyclin B3 mRNA 在性腺中的含量最高, 而在外套膜中的含量最少, 前者是后者的92 倍左右; 对处于不同发育阶段性腺中cyclin B3 基因表达分析结果显示, 性腺中cyclin B3 mRNA 含量随性腺发育程度的提高而提高, 这与其在细胞分裂和减数分裂中的作用相符, 同时也有助于满足早期胚胎发育时期旺盛的细胞分裂对cyclin B3 蛋白需求。 |
| 关键词: 长牡蛎(Crassostrea gigas) 细胞周期调控 cyclin B3 性腺 Real Time PCR |
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| 基金项目:国家“863”计划资助项目(2006AA10A401); 国家“973”计划资助项目(2010CB126401); 国家公益性行业(农业)科研专项(3-53) |
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| Molecular cloning and characterization of the key regulator of cell cycle cyclin B3 in Pacific Oyster (Crassostrea gigas), and its role in gonad development |
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| Abstract: |
| The full length cDNA sequence of cyclin B3 was cloned from Pacific oyster (Crassostrea gigas) for the first time. Cyclin B3 cDNA was 2383 bp in length, containing a 1293bp CDS that encoded a peptide of 430 amino acids with a calculated molecular weight of 47.8 kD. Multiple alignment and converted domain analysis showed that this peptide was a homolog of cyclin B3. The gDNA sequence of cyclin B3 contained 10 exons and 9 introns, which was consistent with the position in evolution of C. gigas. Expression of cyclin B3 gene was found in all tissues, but also showed a very strong tissue-specific feature; the amount of cyclin B3 in gonad was the highest among tissues, 100 times of mantle, the lowest expression tissue. The mRNA amount of cyclin B3 in gonad increased with the maturation of gonad. The expression implied the important role of cyclin B3 in mitosis, meiosis and early development of embryo. |
| Key words: Pacific oyster (Crassostrea gigas) cell cycle regulation cyclin B3 gonad Real Time PCR |
