| 摘要: |
| 采用鼠尾藻嫩芽为实验材料, 研究了不同光强(25～220 μmol/(m2·s))、温度(5～34℃)和盐度(0～60)对其叶绿素荧光参数的影响。测定的叶绿素荧光参数包括光系统II 最大荧光产量(Fv/Fm)、最大潜在相对电子传递速率(RrET,max)、快速光曲线的初始斜率等。结果表明: (1)鼠尾藻嫩芽易受强光、高温胁迫, 在强光照射下, 其Fv/Fm值明显降低, 温度大于30℃时对其光合作用有显著影响; (2)5℃低温处理1 h 或0～60 盐度处理9 h 对光系统Ⅱ均有明显影响, 但24 h 恢复后, 除了盐度60 组, 其他组的叶绿素荧光参数均可基本恢复正常。初步分析了鼠尾藻嫩芽的抗逆生理, 可为鼠尾藻的人工栽培提供参考。 |
| 关键词: 鼠尾藻(Sargassum thunbergii) 胁迫条件 叶绿素荧光参数 |
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| 基金项目:国家“863”计划项目(2006AA10A416, 2012AA100813); 公益性行业(农业)专项(200903030) |
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| Effects of light intensity, temperature and salinity on newborn branches of Sargassum thunbergii evaluated with chlorophyll fluorescence assay |
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| Abstract: |
| The effects of different light intensity (25～220 μmol/(m2·s)), temperatures (5～34 ) and different ℃ erent salinities (0～60) on chlorophyll fluorescence parameters of newborn branches of Sargassum thunbergii were studies in this paper. The optimal chlorophyll fluorescence quantum yield of photosystem II (Fv/Fm), maximum relative electron transport rate (RrET,max) and initial slope of rapid light curve were determined. The results show: (1) The newborn branch of S. thunbergii suffered from high intensity light and high temperature stress easily and the Fv/Fm decreased significantly under high intensity light. The photosynthesis was remarkably affected when the temperature was higher than 30℃. (2) One hour treatment at 5℃ or nine-hour treatment at 0～60 salinity affected the photosystem II obviously. However, the chlorophyll fluorescence parameters could nearly recover to normal level after 24 hours under standard culture condition except those in 60 salinity group. The preliminary analysis of the newborn branches of S. thunbergii about resistance physiology could provide reference for the artificial cultivation of S. thunbergii. |
| Key words: Sargassum thunbergii stress condition chlorophyll fluorescence parameters |
