| 摘要: |
| 利用线粒体COI 基因序列片段与ISSR 标记方法, 研究江苏海域大黄鱼(Pseudosciaena crocea)野生群体(JS)的遗传多样性水平, 并比较其与浙江(ZJ)、福建(FJ)养殖群体的遗传差异。结果表明: (1)扩增COI 基因序列片段625bp, 3 个群体65 个序列中有15 个单倍型, 群体间有3 个共享单倍型; JS 野生群体有9 个独享单倍型, 另有2 个单倍型与ZJ 群体共享; 群体间的遗传分化水平很低, 其中: JS 与ZJ、FJ的遗传分化系数(Gst)分别为-0.0196 和0.02115, FJ 与ZJ 的Gst为-0.0125; (2)筛选出10 个ISSR 标记多态引物, 分析得出JS、ZJ 及FJ 的多态位点百分率分别为74.59%、63.93%及59.02%; 群体间的Nei's 遗传一致度(I)为0.9246～0.8310。其中JS 与ZJ、FJ 的I分别为0.9246 和0.8310, FJ 与ZJ 的I为0.8826。 |
| 关键词: 大黄鱼(Pseudosciaena crocea) COI ISSR 遗传多样性 群体遗传差异 |
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| 基金项目:“十一五”国家科技支撑计划重大项目(2006BAD09A01) |
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| Study of genetic diversity of wild and culture populations of Pseudosciaena crocea using two molecular markers |
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| Abstract: |
| Large yellow croaker, Pseudosciaena crocea Richardson 1846, is a commercially important marine fish and now is very scarce. The fish could be found only occasionally in the Lvsu Fisheries Region in Jiangsu(JS) province, and no researches were carried out about this wild population. In the present study, partial mitochondrial cytochrome oxidase I gene (COI) gene sequence and inter-simple sequence repeat (ISSR) markers were used to investigate the genetic diversity of this wild population and two cultivated populations from Zhejiang(ZJ) and Fujian(FJ) province. The results were as follows: (1) The amplified fragment was 625 bp, revealing 15 haplotypes and three haplotypes of which were shared by these populations. The JS wild population has nine unique haplotypes and two haplotypes shared with the ZJ population. Nei's coefficient of differentiation(Gst) between populations were -0.0196 (JS - ZJ), 0.02115(JS- FJ) and -0.0125(FJ-ZJ), respectively; (2) Ten informative and reliable primers were selected to investigate the genetic diversity. The proportation of polymorphic loci of JS, ZJ and FJ populations were 74.59%, 63.93% and 59.02%, respectively. Nei's genetic identity between populations were 0.9246(JS-ZJ), 0.8310(JS-FJ) and 0.8826(FJ-ZJ), respectively. |
| Key words: Pseudosciaena crocea COI partial sequence ISSR genetic diversity population genetic differentiation |
