| 摘要: |
| 栉孔扇贝(Chlamys farreri)是我国北部沿海一个非常重要的养殖种类。核苷二磷酸激酶(NDPK)是一类广泛存在、高度保守, 在细胞能量和信息传递中具有重要作用的酶。作者利用BAC-FISH 技术将两个包含栉孔扇贝NDPK 基因的BAC 克隆定位到染色体同一位置上, 该结果验证了物理图谱相关contig 组装的可靠性, 同时NDPK 基因的染色体定位将对深入研究该基因的结构、功能以及应用于生产实践提供理论支撑。本研究结果将对栉孔扇贝染色体的深入研究以及染色体鉴别、图谱整合等工作提供重要参考。 |
| 关键词: 栉孔扇贝(Chlamys farreri) 核苷二磷酸激酶 荧光原位杂交 BAC 染色体鉴别 |
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| 基金项目:国家自然科学基金项目(30730071, 30972245); 农业部农业科技成果转化资金项目(2010GB24910700) |
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| Chromosomal localization of NDPK gene in Zhikong scallop Chlamys farreri using BAC-FISH |
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| Abstract: |
| Zhikong scallop(Chlamys farreri) is one of the most economically important aquaculture species in China. Using the technology of BAC-FISH, we localized the BAC clones containing NDPK gene in a pair of homologous chromosomes. This result proved the reliability of the contig assembling of the C. farreri physical map, at the same time, the localization of NDPK gene in chromosome provided further support for research of structures, functions and the application of the gene. This research is the practice of localizing genes of low copies. The result will provide reference to further research and characterization of C. farreri chromosomes, and will facilitate the integration of the physical map, linkage map and cytogenetics map of this species. |
| Key words: Zhikong scallop, Chlamys farreri, NDPK gene, BAC-FISH, Chromosomal identification |
