| 摘要: |
| 扁浒苔(Ulva compressa)能够导致绿潮灾害, 对其开展快速检测技术研究是预防和控制其危害的重要技术手段。根据石莼属不同种类核糖体基因转录间隔区域(ITS)的序列设计了一对检测扁浒苔的特异性引物, 并对其反应条件和体系进行了优化。PCR 特异性检测结果表明, 供试扁浒苔均扩增出与预期大小相一致的330 bp 产物, 而对缘管浒苔(Ulva linza)、浒苔(U. prolifera)、曲浒苔(U. flexuosae)、孔石莼(U. pertusa)的检测结果全为阴性。PCR 灵敏度试验表明, 该PCR 体系最低可以检测到10 pg 的扁浒苔DNA 模板。本快速检测方法为扁浒苔的早期识别与有效治理提供了科学依据, 对相关扁浒苔产业和生态学等研究也具有一定参考意义。 |
| 关键词: 扁浒苔(Ulva compressa) PCR 检测 |
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| 基金项目:中国检验检疫科学技术研究院基本科研业务费专项资金资助项目(2009JK002); 国家质检总局科研项目(2011IK181); 浙江省重点科技创新团队项目(2010R50029) |
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| Rapid detection of Ulva compressa by PCR |
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| Abstract: |
| Ulva compressa is one of causal agent for green tide. Rapid detection of U. compressa is important to prevent and control green tide. In this study, a pair of primers was designed for PCR diction of U. compressa according to the differential region of ITS rDNA of Ulva sp．The protocols for PCR amplification was optimized. The PCR detection can differentiate U. compressa from other Ulva algaes, including U. linza, U. prolifera, U. flexuosae, and U. pertusa. The detection sensitivity was 10 pg of algae genome DNA per PCR reaction. The specific PCR can be applied to rapid detection of U. compressa. |
| Key words: Ulva compressa PCR detection |
