摘要: |
通过室内半静态暴毒实验, 在铜、铅、镉三种重金属的单一胁迫与两两联合胁迫条件下, 研究了繁殖期泥蚶(Tegillarca granosa)对重金属的富集规律。结果表明: 泥蚶对单一Cu2+、Pb2+、Cd2+的生物富集系数(BCF)分别为210.16~1 178.66、128.15~603.84 和198.84~659.37; 联合重金属胁迫条件下, Cd2+对Cu2+的富集具有一定的拮抗作用; Cd2+和Cu2+在泥蚶体内以可溶形式存在, 而Pb2+以不溶形式存在;Cu2+和Cd2+对泥蚶富集Pb2+有一定的协同效应; 砂滤海水中的暂养的泥蚶, 其体内的重金属含量除Cu2+外都较为稳定。 |
关键词: 泥蚶(Tegillarca granosa) 重金属 联合胁迫 生物富集系数 |
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基金项目:国家科技支撑计划项目(2009BADB7B02); 国家现代农业产业技术体系项目(nycytx-47) |
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Accumulation characteristics of heavy metals in Tegillarca granosa under singleor combined stress exposure |
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Abstract: |
A semi-static exposure toxicity test was conducted under laboratory-controlled conditions to study the accumulation characteristics and mechanisms of heavy metals (Cu, Pb, and Cd) in brood stock of Tegillarca granosa in the presence of single or combined heavy metals. The bioconcentration factors (BCF) of Cu, Pb, and Cd were 210.16~1 178.66, 128.15~603.84 and 198.84~659.37 respectively. The value of Cu in T. granosa was higher than those of Pb and Cd; In the presence of combined heavy metal, a competitive adsorption relationship existed between Cu and Cd. The localization of Cu and Cd was in soluble fraction, whereas Pb was located in insoluble fraction. A synergistic relationship existed between Cu and Cd; and the accumulation of Cu was related to process of breed and lipid; the accumulation of heavy metals was closely bound to the binding sites and metal-binding proteins, therefore, heavy metals except Cu were a stable condition in T. granosa with time expansion in sand filtered seawater. |
Key words: Tegillarca granosa heavy metal combined stess bioconcentration factors |