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| 刺参肠道与养殖池塘环境中异养细菌和弧菌数量周年变化 |
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李 彬1, 荣小军1, 廖梅杰1, 张 正1, 王印庚1, 王 岚1, 刘智超1,2, 薛太山3
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1.中国水产科学研究院 黄海水产研究所, 青岛市海水鱼类种子工程与生物技术重点实验室;2.中国海洋大学;3.青岛瑞滋海珍品发展有限公司
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| 摘要: |
| 2008 年9 月～2009 年10 月, 对刺参肠道与养殖环境中异养细菌和弧菌周年变化进行了初步研究。结果表明: 刺参肠道与养殖环境中(水体、底泥、附着基)异养细菌数(HPC)、弧菌数(VBC)均呈现出明显的季节变化, 肠道中HPC、VBC 分别为1.85×105～2.17×109 CFU/g、4.1×104～2.2×108 CFU/g, 水体中HPC、VBC 分别为90～4.67×105CFU/mL、0～5.3×103CFU/mL, 底泥中HPC、VBC 分别为9.80×104～6.72×106CFU /g、1.01×104～5.75×105CFU/g, 附着基中HPC、VBC 分别为2.78×105～2.57×107CFU /g、4.6×104～1.31×107 CFU /g, 夏、秋两季细菌总数较高, 冬、春低温季节细菌数量显著下降。通过VBC 与HPC 的比值可以看出, 在冬、春季细菌总数较低的季节, 弧菌与异养细菌数量比例较高,最高值达到: 43.8%、43.2%, 通过对优势菌的分离鉴定, 冬、春两季灿烂弧菌(Vibrio splendidus)均占优势, 这可能是造成冬、春两季刺参发病的诱因之一。本文通过研究刺参肠道与养殖环境中异养细菌和弧菌的变化规律, 以期为刺参的健康养殖提供有益参考。 |
| 关键词: 刺参 肠道 环境 细菌数量 周年变化 |
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| 基金项目:国家自然科学基金项目(30901120); 国家高技术研究发展计划项目(2006AA100313); 中央级公益性科研院所基本科研业务费专项;山东省农业良种工程重大课题 |
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| Annual changes of total heterotrophic bacteria and Vibrios in the intestine of Apostichopus japonicus and its culture pond |
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| Abstract: |
| Annual changes of total heterotrophic bacteria and Vibrios in intestine of Apostichopus japonicus and its culture environment were studied from September 2008 to Novemver 2009. We found that the number of bacteria estimated by HPC (Heterotrophic Plate Count) and VBC (Vibrio bacterial Count) had significant seasonal changes in the intestine of A. japonicus and the cultured environment. HPC and VBC in the intestine of A. japonicus ranged from 1.85×105 to 2.17×109 CFU/g and from 4.1×104 to 2.2×108 CFU/g, respectively. HPC and VBC in cultured pond water ranged from 90 to 4.67×105 CFU/mL and from 0 to 5.3×103 CFU/mL, respectively. HPC and VBC in the pond sediment ranged from 9.80×104 to 6.72×106 CFU /g and from 1.01×104 to 5.75×105 CFU/g, respectively. HPC and VBC in cultch sediment ranged from 2.78×105 to 2.57×107 CFU /g and from 4.6×104 to 1.31×107 CFU/g, respectively. The total bacterial count in summer or autumn was higher than other seasons. The ratios of VBC to HPC were also calculated and indicated that the proportion of Vibros reached 43.8% and 43.2% in winter and spring. Although HPC in winter and spring were lower than thoese of summer and autum, the proportions of Vibro were higher in winter and spring than that of summer and autumn, potentially being the reason of high disease incidence during winter and spring. Our results could be useful to healthy culture of sea cucumber. |
| Key words: Apostichopus japonicus Intestine Environment Bacteria Annual change |