| 摘要: |
| 醇沉法制得低聚壳聚糖美拉德反应8、16 h 以及24 h 的衍生物, 分别记为CC-8、CC-16 以及CC-24。对3 种衍生物进行红外表征和分子量测定, 并研究其对超氧阳离子O-2、DPPH 的清除能力以及还原能力。结果显示: 反应体系pH 呈下降趋势; UV-Vis 光谱在280 nm 波长处吸收峰有明显增强, 反应16 h 后增长缓慢; 在343 nm 的激发波长和420 nm 发射波长下的荧光强度明显增高, 反应16 h 后开始下降; 3 种衍生物均保留着低聚壳聚糖的特征吸收峰; 其对O-2、. DPPH 的清除能力以及还原能力均得到显著提高, 且CC-16 抗氧化能力最好。 |
| 关键词: 低聚壳聚糖 美拉德反应 抗氧化性能 |
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| 基金项目:“十二五”国家支撑计划项目(2012BAD38B09); 上海市科委工程中心建设项目(11DZ2280300) |
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| The Maillard reaction of chitosan oligosaccharide and the antioxidative activities of its derivatives |
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| Abstract: |
| Three kinds of chitosan oligosaccharide derivatives prepared through Maillard reaction by heating chitosan oligosaccharide for 8, 16 and 24 hours were named CC-8, CC-16 and CC-24, respectively. The pH, absorbance and fluorescence were determined during the reaction. Chitosan oligosaccharide derivatives were characterized by Fourier transform infrared (FTIR) spectroscopy and their molecular weights were determined by Gel permeation chromatograph (GPC). Their antioxidative activity was investigated by employing various established in vitro systems, such as superoxide anion, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging, and their reducing capacity was also studied. The results showed that the pH value of the reaction system decreased during Maillard reaction. The UV-Vis absorbance increased significantly at 280 nm and this increase slowed down after 16 hr. The fluorescence intensity significantly increased with Ex at 343 nm and Em at 420 nm and also slowed down after 16 hr. All the three derivatives kept the characteristic absorption peak of chitosan oligosaccharide. Their antioxidative and reducing capacities were both much stronger than those of the chitosan oligosaccharide and CC-16 has the strongest antioxidative activity. |
| Key words: chitosan oligosaccharide Maillard reaction antioxidative activity |
