| 摘要: |
| 采用聚合酶链式反应限制性片段长度多态性分析(PCR-RFLP)技术对劳盆地热液区TVG9 站位沉积物中细菌、古菌多样性进行了调查, 并构建其细菌、古菌种群的16S rRNA 基因文库。研究结果表明, 该站位细菌包含变形杆菌(Proteobacteria)、硝化螺旋菌(Nitrospira), 疣微菌(Verrucomicrobia)等3 个类群, 其中变形杆菌占据优势地位, 由Alphaproteobactria, Gamaproteobactria, Deltaproteobactria等3 个亚群组成。古菌包含泉古菌(Crenarchaeota)和广古菌(Euryarchaeota), 其中泉古菌占优势,由MGⅠ(Marine GroupⅠ)和MCG(Miscellaneous Crenarchaeotal Group)两类群组成, 而广古菌主要由MBGE(Marine Benthic Group E)组成。旨在揭示深海热液区的微生物多样性, 为生态环境的研究提供理论支持。 |
| 关键词: 劳盆地 热液喷口 沉积物 微生物多样性 聚合酶链式反应限制性片段长度多态性分析(PCR-RFLP) |
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| 基金项目:国家自然科学基金(91028005) |
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| Phylogenetic analysis of microbial diversity of the sediment from Lau basin |
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| Abstract: |
| The bacterial and archaeal diversity of the deep-sea sediment collected at Lau Basin TVG9 sites were analyzed using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 16S rRNA gene sequences. The 16S rRNA gene libraries were constructed. The results showed that there were three phyla in the Bacteria domain, including Proteobacteria, Nitrospira, Verrucomicrobia, and the phylum Proteobacteria is predominant, which has three sub-groups including Alphaproteobactria, Gamaproteobactria and Deltaproteobactria. In the Archaea domain, Crenarchaeota dominates over Euryarchaeota. There were two divisions in the Crenarchaeota kingdom, MGⅠ(Marine Group Ⅰ)and MCG(Miscellaneous Crenarchaeotal Group). The MBGE(Marine Benthic Group E) is the dominant group in the Euryarchaeota. This study has uncovered the microbial diversity in the deep-sea hydrothermal region, and provided more evidence for the ecology environmental research. |
| Key words: Lau Basin hydrothermal vent sediment microbial diversity polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) |
