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海洋细菌LP621 右旋糖苷酶分批发酵动力学研究
葛 亮1,2, 房耀维2, 吴文惠1, 王淑军2, 吕明生2, 焦豫良2, 刘 姝2
1.上海海洋大学 食品学院;2.淮海工学院 海洋学院
摘要:
对海洋细菌Pseudoalteromonas tetraodonis LP621 右旋糖苷酶进行了50L 发酵罐分批发酵的代谢特性的研究。结果表明LP621 右旋糖苷酶合成和菌体生长呈部分生长偶联型。据分批发酵试验结果采用Logistic 方程、Luedeking-Piret 方程建立了描述菌株生长、产酶以及葡萄糖底物消耗分批发酵动力学模型。动力学模型计算值结果与实验值拟合良好, 较好反映了菌株右旋糖苷酶分批发酵过程的动力学特征。
关键词:  右旋糖苷酶  海洋细菌  发酵动力学模型  Logistic 方程  Luedeking-Piret 方程
DOI：
分类号:
基金项目:国家高技术研究发展计划项目(2011AA09070302)
Kinetics analysis for dextranase of Pseudoalteromonas tetraodonis LP621 by liquid fermentation
Abstract:
The kinetics for dextranase of marine bacteria Pseudoalteromonas tetraodonis LP621 by liquid fermentation was studied. The results showed that the dextranase production was partially associated with the growth of LP621. Based on the result of batch fermentation, the kinetic models for cell growth, dextranase formation, sugar consumption were set up using Logistic equation and Luedeking-Piret equation. The calculated results of models well matched the experimental data. The model equations could well reflect the dextranase fermentation process and kinetic characteristic.
Key words:  dextranase  marine bacteria  fermentation kinetic model  logistic equation  luedeking-Piret equation
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