| 摘要: |
| 通过PCR扩增获得了3 科5 属6 种中国黄渤海海域的鳚亚目(Blennioidei)鱼类的线粒体16S rRNA基因序列片段约669bp 碱基, 结合来自GenBank 的5 种鳚亚目其他科鱼类的相应基因片段, 并以眼斑雪冰鱼(Chionodraco rastrospinosus)为外群, 生成供系统发育分析的序列矩阵, 利用MEGA 4.0 软件分析序列的碱基组成、差异百分比、转换／颠换值等, 应用最大简约法(MP)和邻接法(NJ)构建系统发育树。结果显示: 在鳚亚目鱼类的16S rRNA 片段生成的序列矩阵中发现有碱基的插入缺失现象, 共有207 bp 变异位点, 转换／颠换值为0.8, 碱基平均差异为3.36; 支持绵鳚(Enchelyopus elongates)归于鳚亚目绵鳚科(Zoarcidae), 繸鳚(Azuma emmnion)归于鳚亚目线鳚科(Stichaeidae); 方氏云鳚(Enedriasfangi)和云鳚(Enedrias nebulosus)种间遗传距离只有0.01, 亲缘关系最近。 |
| 关键词: 鳚亚目(Blennioidei) 线粒体DNA 16S rRNA 基因序列 系统发育 |
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| 基金项目:辽宁省重大科技公关项目(2008203002);辽宁省教育厅计划项目(2009A175) |
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| Molecular phylogenetic relationship of blennioidei based on partial sequence of mitochondrial 16S ribosomal RNA gene |
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| Abstract: |
| The 16S ribosomal RNA partial sequences of 6 species within 5 genera from Blennioidei in China yellow and bohai seas were amplified using PCR technique. Approximately, 669 bp gene fragments were obtained. These data were combined with other homologous sequences of 5 species from Blennioidei, which were downloaded from the GenBank. Chionodraco rastrospinosus were designed as outgroup. Nucleotide composition frequencies, P-Distance genetic distance and transition/transversion ratios were analyzed with MEGA 4.0 software. Molecular phylogenetic trees were constructed by the maximum parsimony (MP) and neighborjoining (NJ) methods. There were insertions and deletions in the aligned base pairs. Two hundred and seven bp variable sites were found in the analysis matrix. The average ts/tv ratio and average percentage divergence among all pairwise comparisons were 0.8 and 3.36, respectively. According to the partial sequence of mitochondrial 16S ribosomal RNA gene, the classification system of Blennioidei was acceptable, which is classified as Pholidae, Zoarcidae, Stichaeidae, Clinidae, Blenniidae, Chaenopsidae and Tripterygiidae. Enedrias fangi and Enedrias nebulosus had a close relationship, as the genetic distance between the two species of partial 16S ribosomal RNA gene was only 0.01. |
| Key words: Blennioidei mitochondrial DNA (mtDNA) 16S ribosomal RNA (16S rRNA) molecular phylogeny |
