| 摘要: |
| 利用叶绿素荧光技术对坛紫菜(Porphyra haitanensis)叶状体营养细胞和生殖细胞的光能利用特性进行了比较。结果表明: 坛紫菜叶状体营养细胞和雌性生殖细胞的实际量子效率(ΔF/Fm′)差异不明显,但显著高于雄性生殖细胞; 快速光曲线测定表明雌、雄藻体营养细胞的最大相对电子传递速率(rETRmax)相近, 但显著高于雄性生殖细胞; 不同生殖细胞半饱和光强(Ik)无显著差异; 在生长光强下, 营养细胞和雌性生殖细胞rETR、光化学猝灭(qP)和非光化学猝灭(NPQ)差异不明显, 而雄性生殖细胞rETR、qP 等荧光参数均显著低于营养细胞和雌性生殖细胞。本文表明坛紫菜叶状体营养细胞和雌性生殖细胞具有较高的光能利用能力, 能够将吸收的光能多数用于电子传递, 而雄性生殖细胞对光能的利用能力较低。 |
| 关键词: 坛紫菜(Porphyra haitanensis) 生殖细胞 营养细胞 叶绿素荧光 |
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| 基金项目:国家科技支撑计划项目(2011BAD13B00); 国家海洋局公益性行业专项(201105023); 农业部公益性专项(2009030 国家自然科学基金项目(40906085) |
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| Compare of chlorophyll fluorescence characteristics between vegetative and germ cells of Porphyra haitanensis |
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| Abstract: |
| Light utilization characteristics of vegetative and germ cells of Porphyra haitanensis were compared by technique of chlorophyll fluorescence. The results showed that the actual photochemical efficiency of PSII (ΔF/Fm′) of the vegetative cells and female cells exhibited only a little difference, but they were both significantly higher than that of the male cells. The maximum relative electron transport rate (rETRmax) of the vegetative cells and female cells were both significantly higher than that of the male cells. There was no significant difference in half saturating irradiance (Ik) between the vegetative and germ cells. The vegetative cells and female cells had similar rETR, photochemical quenching (qP) and non-photochemical quenching (NPQ) under the growing light intensity, which were higher than those of the male cells. These results suggested that the vegetative cells and female cells of P. haitanensis could use light efficiently and most of absorbed light energy was used for photosynthetic electron transport, but the male cells had much lower light energy use efficiency. |
| Key words: Porphyra haitanensis germ cell vegetative cells chlorophyll fluorescence |
