| 摘要: |
| 为查明波纹巴非蛤(Paphia undulata)幼贝最适生长条件, 采用响应面法研究了温度、盐度和密度交互作用对波纹巴非蛤幼贝生长的影响。利用Box-Behnken中心组合进行3因素3水平的实验设计(3因素为温度(A), 盐度(B), 密度(C); 3水平为－1, 0, 1), 以幼贝生长率为响应值, 以A(26℃, 28℃, 30℃)、B(28, 30, 32)和C(0.56, 1.4, 2.24 个/cm2)为影响因子。实验结果表明: (1)盐度对幼贝生长影响最显著(P<0.05), 3种生态因子对幼贝生长率影响程度大小顺序是: B>A>C; (2)通过SAS软件分析, 得出波纹巴非蛤幼贝最适生长环境组合是温度为26.93℃、盐度为31.39、密度为1.12个/cm2, 理论最佳生长率为 41.47%。为验证响应面法预测结果的准确性, 以预测结果为实验条件, 相同实验时间后, 得到的生长率为42.30%, 结果与理论预测值相近, 响应面方法可靠。 |
| 关键词: 响应面法 波纹巴非蛤(Paphia undulata)幼贝 生长率 |
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| 基金项目:福建省科技厅科技重大项目(2010N5007)； 国家海洋局海洋公益行业科研专项资助(201205021) |
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| The effect of temperature, salinity and density on growth of Paphia undulata youth analyzed by response surface methodology |
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| Abstract: |
| In order to explore the optimum growth conditions for Paphia undulata youth, response surface methodology was used to study the effect of temperature, salinity and density on growth of Paphia undulata youth. The experiment was designed according to Box-Behnken central composite design in three factors and three levels, and temperature (26℃, 28℃, 30℃), salinity (28, 30, 32), density (0.56,1.4,2.24ind/cm2) were selected as affecting factors. The results showed that the effect of salinity on the growth rate was the most significant (P<0.05), and the order of three ecological factors effects on the youth growth was salinity (B) > temperature (A) > density (C). By means of SAS software, the optimum growth environment condition of Paphia undulata youth was confirmed as follows: temperature 26.93℃, salinity 31.39 and density 1.12ind/cm2, and theoretical optimal growth rate was 41.47%. After experimental verification, the growth rate was 42.30%. To verify the accuracy of the results predicted by RSM, the real growth rate was 42.30% under the theoretical condition, suggesting RSM was reliable. |
| Key words: response surface methodolody Paphia undulata larvae growth rate |
