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| 低盐度形成的微生物膜对厚壳贻贝稚贝附着的影响 |
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杨金龙1,2,3, 李 响1, 王 冲4, 凌 云1, 包卫洋5, 沈和定1,2,3, 李家乐1,2,3
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1.上海海洋大学 农业部淡水水产种质资源重点实验室;2.上海市水产养殖工程技术研究中心;3.上海高校知识服务平台上海海洋大学水产动物遗传育种中心;4.滨州市海洋与渔业研究所;5.扬州大学海洋科学与技术研究所
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| 摘要: |
| 为研究环境因子在厚壳贻贝(Mytilus coruscus)稚贝附着过程中的调控作用, 作者探讨了低盐度对微生物膜生物构成、群落结构的影响及所形成微生物膜对厚壳贻贝稚贝附着的影响。在实验室条件下, 研究微生物膜的日龄与干质量、细菌密度和硅藻密度、叶绿素a含量的关系及其对厚壳贻贝稚贝附着的影响。通过DGGE指纹图谱技术对微生物膜中的细菌群落结构多样性进行了分析。研究发现,盐度13和23时形成的微生物膜能有效促进厚壳贻贝稚贝的附着, 且盐度23、28d时稚贝附着率最高, 达到72%。相关性分析表明, 微生物膜的诱导活性与盐度、干质量、细菌密度、硅藻密度、日龄呈显著正相关性, 与叶绿素a无相关性。微生物膜的干质量、附着细菌密度及底栖硅藻密度明显随着日龄的增加而增加, 叶绿素a含量与微生物膜日龄无显著相关性。细菌群落在厚壳贻贝稚贝附着过程中发挥重要调控作用。 |
| 关键词: 厚壳贻贝(Mytilus coruscus) 稚贝 微生物膜 盐度 附着 |
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| 基金项目:国家自然科学基金项目(31101885); 上海市科委重点支撑项目(12230502100) |
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| Settlement of the mussel Mytilus coruscus plantigrades in response to biofilms formed on low salinities |
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| Abstract: |
| The influence of low salinity formed biofilms on the settlement of the plantigrade Mytilus coruscus was investigated in the laboratory. Various aspects of biofilms were examined including age, dry weight, chlorophyll a concentration, bacterial and diatom densitie. The diversity of bacterial community structure was analyzed by fingerprint technique DGGE. It is found that the settlement of M. coruscus was promoted by the biofilms which are formed when the salinity is 13 or 23. Furthermore the adhesive rate reached maximum value 72% when the salinity is 13 or 23. It is indicated that the inducing activity of biofilm was positively correlated with salinity, biofilm age, dry weight and bacterial and diatom densities, but uncorrelated with chlorophyll a concentration. The dry weight, bacterial densities and diatom densities in biofilm increased with biofilm age, but chlorophylla was uncorrelated with biofilm age. Overall, this investigation indicates that bacterial structure may play an important role in the adhesion of the plantigrade M. coruscus, which can be induced by low salinity formed biofilms. |
| Key words: plantigrade Mytilus coruscus biofilms salinity settlement |