| 摘要: |
| 研究了温度和光生物反应器的光径对微绿球藻(Nannochloropsis oculata Hibberd)生长及细胞内几种营养成分含量的影响。结果表明, 温度对微绿球藻的生长有显著影响(P<0.05), 对几种营养成分的含量有极显著影响(P<0.01), 在28℃的培养条件下, 细胞生长速度最快, 此时蛋白质含量最高, 为5.66%, 多糖含量最低, 仅为1.47%, 但最大生物产量却是在30℃的条件下获得, 总脂与叶绿素a 的含量随温度的升高而降低, 类胡萝卜素含量随温度升高而增大。光生物反应器的光径对微绿球藻的生长和总脂、可溶性蛋白、色素的含量有极显著影响(P<0.01), 对多糖含量有显著影响(P<0.05), 比生长速率、最高细胞密度和单位体积产量均随光径的增大而减小, 单位面积产量随光径的增大而增大; 总脂、可溶性蛋白和多糖的含量随光径的增大而降低, 叶绿素和类胡萝卜素含量随光径增大而增大, 类胡萝卜素含量的增大趋势比叶绿素更明显。 |
| 关键词: 微绿球藻(Nannochloropsis oculata Hibberd) 温度 光径 蛋白质 多糖 总脂 色素 |
| DOI：10.11759/hykx20120419002 |
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| 基金项目: |
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| Effect of temperature and optical path on growth rate and accumulation of nutrients of Nannochloropsis sp. |
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| Abstract: |
| The effect of temperature and optical path of flat plate glass reactors on growth rate and organics contents of Nannochloropsis sp. was investigated, using a range of optical paths from 5 to 20 cm. The results showed that the temperature had a significant effect on the growth of Nannochloropsis (P<0.05), and a very significant effect on the contents of mutrients (P<0.01). The cell growth had a highest speed at 28℃, when the content of soluble protein reached the maximum and that of polysaccharides reached the minimum. The biomass yield reached the maximum at 30℃. The contents of lipid and chlorophyll decreased with the increase of temperature, while the content of carotenoid increased with the increase of temperature. The optical path of photobioreactors had a very significant effect on the growth of Nannochloropsis (P<0.01). Specific growth rate, maximal cell density (107 cell/mL) and yield per unit volume (mg /(L·d) ) decreased with increase in optical path. Yield per unit area (g /(m2·d) ) increased with increased optical path. The contents of lipid, soluble protein, and polysaccharides decreased with increased optical path, but the contents of chlorophyll and carotenoid increased with increased optical path. The increase trend of carotenoids was more obvious than that of chlorophyll. In summary, the optimal optical path determines the optimal culture density, as well as yield per unit volume and the productivity of organics. Under our conditions the optimal optical path for culturing Nannochloropsis to accumulate lipid, protein and polysaccharide in plate reactors was 5 cm, the optimal optical path for accumulating pigments was 20 cm. |
| Key words: Nannochloropsis sp. temperature optical path protein polysaccharide lipid pigments |
