| 摘要: |
| 为了探寻连续降温对大菱鲆(Scophthalmus maximus)成鱼血清、肝脏及肌肉能量代谢等指标的影响, 作者将大菱鲆从正常养殖水温18℃快速、连续降温至1℃, 并在18℃、13℃、8℃、5℃、3℃和1℃共计6 个温度点取血采样, 分别测定血清中的总蛋白(TP)、白蛋白(ALB)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、总胆固醇(TC)、血糖(GLU)以及肝脏中的糖原和肌肉中的乳酸等指标。结果发现, 随着温度降低, 大菱鲆的血清TP 浓度呈先下降后升高的趋势, 各实验组与对照组无显著差异(P>0.05), 但在13℃和3℃之间出现显著性差异(P<0.05); 血清ALB 浓度则呈现升高趋势, 在3℃时显著高于1℃(P<0.05); 血清TC 浓度呈现升高趋势, 且3℃时显著高于18℃(P<0.05); GLU 浓度呈先降低后升高趋势, 且8℃、5℃、3℃实验组浓度显著高于其余实验组(P<0.05); 尿素浓度呈先降低后升高再降低趋势, 且8℃时显著高于1℃(P<0.05); 肝糖原浓度呈先降低后升高趋势, 且8、5、3℃实验组浓度显著高于其余实验组(P>0.05); 肌肉中乳酸含量呈先升高后降低趋势, 且5℃时显著高于18℃(P<0.05); 其余指标则无显著性差异(P>0.05)。结果表明: 当温度在18～8℃时, 降温对大菱鲆体内代谢影响不大; 当温度降到8～3℃时, 大菱鲆体内开始出现应激反应;当温度继续降低至1℃时, 大菱鲆体内的各种代谢基本处于停滞状态。综合分析各温度下的代谢指标得出结论, 3～1℃温度区间与大菱鲆的生态冰温点比较接近, 可以在此温度区间附近进一步进行无水保活的探索, 在保证大菱鲆无水运输可行性的基础上优化经济成本。 |
| 关键词: 大菱鲆(Scophthalmus maximus) 连续降温 血清生化指标 肝糖原 肌肉乳酸 能量代谢 |
| DOI：10.11759/hykx20130617001 |
| 分类号: |
| 基金项目:中国科学院知识创新工程资助项目(KZCX2-EW-Q215);山东省科技发展计划课题(2011GGC21007) |
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| Effect of continuous cooling on metabolic function of adult Scophthalmus maximus L. |
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| Abstract: |
| In order to study the effect of continuous cooling on metabolic indices of serum, liver and muscle, the adult turbot was subject to continuous cooling from 18 to 1℃, and 6 individuals were collected at each specific temperature including 18, 13, 8, 5, 3 and 1℃. The contents of liver glycogen, lactic acid of muscle and eight serum biochemical indices were measured including total protein, albumin, glucose, total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, triglyceride and urea. The results showed that the total protein had significant difference between 13 and 3℃, albumin had significant difference between 3 and 1℃, total cholesterol had significant difference between 18 and 3℃, urea had significant difference between 8 and 1℃, contents of lactic acid in muscle had significant difference between 18 and 5℃. The contents of glucose in the 8, 5 and 1 ℃ groups were significantly higher than those in other groups. The contents of liver glycogen in the 8, 5 and 1℃ groups were significantly higher than those in other groups. In addition, other indices showed no significant difference in the temperature change. The results showed that when the temperature ranged from 18 to 8℃, the metabolism changed quite little, when the temperature decreased from 8 to 3℃, the stress response appeared, when the temperature continuously decreased to 1℃, the metabolism tended to be extremely low, even stoped. According to the analysis of the data, a conclusion can be drawn that temperature between 3 and 1℃ was close to the ecological ice temperature of Scophthalmus maximus, so further exploration of anhydrous transport could be made in this temperature range to lessen the cost. |
| Key words: Scophthalmus maximus lowering the temperature liver glycogen lactic acid of muscle serum biochemical indices |
