| 摘要: |
| 为提高小新月菱形藻(Nitzschia closterium)的产油脂能力、获得油脂含量高的突变株, 利用甲基磺酸乙酯(EMS)对该藻株进行了化学诱变处理, 并对其生物学作用进行了考察。结果显示, EMS 对小新月菱形藻具有较好的诱变作用, 经0.1 mol/L 和 0.05 mol/L EMS 处理后分别得到油脂生产能力较强的突变藻株YA 和YB, 在同等培养条件下两株突变藻单位体积细胞的内叶绿素a、类胡萝卜素、蛋白质、可溶性糖和油脂相对含量分别比对照组提高了30.05%和25.77%、22.15%和19.26%、9.06%和8.27%、48.25%和45.48%、44.06%和12.86% |
| 关键词: 微藻 小新月菱形藻(Nitzschia closterium) 诱变 甲基磺酸乙酯 油脂 |
| DOI：10.11759/hykx20140109001 |
| 分类号: |
| 基金项目:国家863 计划项目(2012AA021706) |
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| The biological effect of the Ethyl Methanesulphonate on Nitzschia closterium |
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| Abstract: |
| In order to obtain the high-lipid producing strains of Nitzschia closterium, the method of chemical mutation with Ethyl Methanesulphonate (EMS) was used, and the biological effects of EMS on the cells of N. closterium were studied. The results showed that the EMS has good mutagenesis effect on the cells of N. closterium, and two different mutant strains of N. closterium YA and YB were obtained after treatment with 0.1 mol/L and 0.05 mol/L dosages of EMS, respectively, which both had stronger oil production capacity. Under the same cultivation condition, the chlorophyll a, carotenoids, protein, soluble sugar contents and lipid relative contents of YA and YB have increased by 30.05% and 25.77%, 22.15% and 19.26%, 9.06% and 8.27%, 48.25% and 45.48%, and 44.06% and 12.86% compared with the CK, respectively. |
| Key words: Microalgae Nitzschia closterium Mutagenesis Ethyl Methanesulphonate lipid |
