| 摘要: |
| 为了解中国南海海洋自生固氮菌的种类, 作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理, 无氮培养基平板初筛后, 对分离获得的细菌固氮酶结构基因nifH进行扩增, 并对其固氮酶活性进行检测, 最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S rDNA 序列分析(GenBank 登录号 KJ627376), 并基于nifH、16S rDNA 系统进化树分析, 确定该菌为一株固氮类芽孢菌(Paenibacillus sp.) NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布, 海洋自生固氮菌的多样性远远超出人们之前的认识。 |
| 关键词: 固氮类芽孢杆菌 固氮酶活性 nifH基因 16S rDNA |
| DOI：10.11759/hykx20140518001 |
| 分类号: |
| 基金项目:山西师范大学生命学院重点项目 (SMYKZ-35) |
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| Isolation and identification of a Paenibacillus sp. Producing nitrogenase activity from South China Sea |
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| Abstract: |
| To understand the diazotrophic microorganisms from the South China Sea, the nitrogen fixation bacteria were isolated from sediments samples, screened and identified. By prescreening in N-free plate after boiling the samples, the nifH gene of isolate NH-1 was analyzed by PCR amplification and the nitrogenase activity was tested by acetylene reduction assay. The strain NH-1 was found to belong to the genus Paenibacillus based on morphological characteristics, physiological and chemical characteristics and 16S rDNA sequencing analysis (GenBank accession No. KJ627376). The isolate NH-1 was identified as Paenibacillus sp. NH-1. This study provides evidence that marine nitrogen-fixing microorganisms are far more diverse than currently recognized. |
| Key words: Paenibacillus sp. nitrogenase activity nifH gene 16S rDNA |
