| 摘要: |
| 浒苔(Ulva prolifera)是构成黄海绿潮的优势种, 通过构建浒苔稳定转化系统, 可以为相关基因功能验证提供有力工具。本文以浒苔原生质体再生苗为材料, 试验了对除草剂草丁膦和6 种抗生素的敏感性, 发现浒苔对氨苄青霉素、卡那霉素、硫酸链霉素、Zeocin 4 种抗生素不敏感, 对氯霉素、潮霉素较敏感, 对除草剂草丁膦极为敏感, 进一步统计得出草丁膦对浒苔的半致死剂量(LD50)和95%可信限,发现敏感性与光周期明显相关。上述结果为进一步筛选转化子、构建浒苔稳定转化系统打下了基础。 |
| 关键词: 浒苔(Ulva prolifera) 基因工程 草丁膦 抗生素 敏感性 光周期 |
| DOI：10.11759/hykx20140423003 |
| 分类号: |
| 基金项目:中国科学院海洋战略性先导科技专项(XDA11020304); 国家863 计划资助项目(2014AA093501); 国家自然科学基金项目(31300-365); 海洋公益项目(201205010); 山东省博士基金项目(2010BSB02009) |
|
| Sensitivity of protoplast-regenerated seedling of Ulva prolifera to phosphinothricin and six kinds of antibiotics |
|
|
| Abstract: |
| Ulva prolifera is the dominant species of green tides in the Yellow Sea, which can accumulate a large amount of biomass in a short period. Establishing the genetic transformation system of U. prolifera can provide a powerful tool for verifying the function of related genes. In this paper, the sensitivity of protoplast-regenerated seedling of U. prolifera to phosphinothricin and six kinds of antibiotics was tested. Results showed that seedlings were not sensitive to ampicillin, kanamycin, streptomycin sulphate and zeocin, weakly sensitive to chloramphenicol and hygromycin, but very sensitive to phosphinothricin. Moreover, the sensitivity to phosphinothricin was significantly correlated with photoperiod. The LD50 and 95% confidence range of phosphinothricin were calculated. These results can lay basis for screening transformants and establishing the genetic transformation system of U. prolifera. |
| Key words: Ulva prolifera genetic engineering phosphinothricin antibiotics sensitivity photoperiod |
