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| 泥蚶5个地理群体遗传多样性的微卫星分析 |
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程雪艳,滕爽爽,肖国强,邵艳卿,张炯明,方军,柴雪良
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温州医科大学,浙江省海洋水产养殖研究所 浙江温州;浙江省海洋水产养殖研究所 浙江温州,浙江省海洋水产养殖研究所,浙江省海洋水产养殖研究所 浙江温州;浙江省海洋水产养殖研究所 浙江温州,浙江省海洋水产养殖研究所,浙江省海洋水产养殖研究所,温州医科大学
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| 摘要: |
| 运用微卫星DNA技术,对浙江温州(ZJ)山东日照(RZ)韩国釜山(KR)广西企沙(GX)海南海口(HN)5个群体进行了17个基因位点的遗传多样性分析。结果显示,17对引物共检测出115个等位基因, 每个位点等位基因数(Na) 2-12个; 每个位点有效等位基因数(Ne)为1.192-7.849; 平均观测杂合度(Ho)为0.430-0.516; 平均期望杂合度(He)为0.573-0.656; 5个群体的平均多态信息含量(PIC)为0.525-0.608; Hardy-Weinberg平衡检验表明, 51.2%的微卫星位点偏离平衡状态(P<0.05)。群体间遗传变异指数Fst 为0.0598, 表明5个泥蚶群体间的遗传变异为5.98%。UPMGA 聚类分析表明, 浙江群体和韩国群体亲缘关系最近首先聚在一起,然后与海南群体聚合；广西群体和山东群体亲缘关系也较近,聚为一支,然后与上面三个群体聚合。 |
| 关键词: 泥蚶 微卫星 遗传多样性 地理群体 |
| DOI： |
| 分类号:Q789 |
| 基金项目:国家高技术研究发展计划(2012AA10A410-1)；浙江省重大科技专项(2012C12907-4)；浙江省科技计划项目(2013F50016)；温州市种子种苗科技创新专项(N20120017)第一作者程雪艳(1992-),女,硕士研究生；研究方向滩涂贝类分子遗传学研究；E-mail: 15658693728@163.com通讯作者柴雪良,研究员,E-mail: cxl-5888@163.com |
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| Microsatellite analysis of genetic diversity in five geographic populations of Tegillarca granosa |
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CHEN Xue-yan,TENG Shuang-shuang,XIAO Guoqiang,SHAO Yan-qing,ZHANG Jiongming,FANG Jun and CHAI Xue-liang
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Laboratory Medicine and Life Science College of Wenzhou Medical University,Zhejiang Mariculture Research Institute;Zhejiang Mariculture Research Institute,Zhejiang Mariculture Research Institute,Zhejiang Mariculture Research Institute;Zhejiang Mariculture Research Institute,Zhejiang Mariculture Research Institute,Zhejiang Mariculture Research Institute,Laboratory Medicine and Life Science College of Wenzhou Medical University
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| Abstract: |
| Genetic diversity in four geographic populations of Tegillarca granosa from Wenzhou, Korea, Rizhao, Qisha and Haikou was analyzed using seventeen polymorphic microsatellite markers in our laboratory. 115 alleles were found in the seventeen microsatellite markers, allele number per locus was 2-12, effective allele number per locus was 1.192-7.849, mean observed heterozygosity were 0.430-0.516 and mean expected heterozygosity were 0.573-0.656. Mean polymorphism information content were 0.525-0.608 in five populations; the Hardy-weinberg balance test showed that microsatellite sites deviated from equilibrium(P<0.05) were 51.2%. The Fst value was 0.0598, suggesting that the genetic variation in the five populations was 5.98%. The UPMGA tree indicated that Zhejiang with Korea population clustered first , and clustered with Hainan population. The Guangxi population clustered with Shandong population into another clade. |
| Key words: Tegillarca granosa microsatellites genetic variability geographical populations |