| 摘要: |
| 以3 种壳色虾夷扇贝(Mizuhopecten yessoensis)(枫叶贝、红贝、白贝)为实验材料, 将在15℃暂养的3 种壳色虾夷扇贝分别驯化至20 , 22 , 24 和26 ℃ 4 个温度梯度(升温幅度为1 ℃/d), 待各处理组达到对应温度梯度后暂养7 d, 测定比较3 种壳色虾夷扇贝的存活率、耗氧率和排氨率、抗氧化酶活力和热激蛋白HSP70 表达等指标。结果表明: 随着温度的升高, 3 种壳色的虾夷扇贝的存活率呈先上升后下降趋势, 其中, 白壳色虾夷扇贝的存活率在同一处理组中最高; 3 种壳色的虾夷扇贝耗氧和排氨率均随温度升高呈现先升高后降低的趋势, 同一温度处理组下白壳色虾夷扇贝的代谢率始终高于红贝和枫叶贝; 另外, 总抗氧化能力(total-antioxidant capacity, T-AOC)、超氧化物歧酶(activity of superoxide dismutase, SOD)、过氧化氢酶(Catalase, CAT)、过氧化物酶(Peroxidase, POD) 4 种免疫酶的活性随温度升高也呈现先升高后降低的趋势, 且同一温度处理组下白壳色虾夷扇贝的免疫酶活力始终高于红贝和枫叶贝; 3 种壳色虾夷扇贝鳃HSP70 的表达模式基本相同, 随着温度的逐渐升高, HSP70 持续表达, 到26℃时表达量最高, 且白壳色扇贝HSP70 的相对表达量最高为5.07。综上, 白壳色虾夷扇贝在高温应激下表现出较强的抵抗和耐受能力, 因此, 可将其作为后期耐高温型虾夷扇贝新品系的重要培育材料。 |
| 关键词: 虾夷扇贝(Mizuhopecten yessoensis) 壳色 高温 免疫酶活力 热激蛋白70(HSP70) |
| DOI：10.11759/hykx20150805001 |
| 分类号: |
| 基金项目:国家海洋公益性行业科研专项(201205031); 国家“948”项目(2011-G12); 国家“863”项目(2012AA10A410); 农业部北方海水增养殖重点实验室开放课题(2014-MSENC-KF-10) |
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| A comparative study of survival, metabolism, immune indicators and HSP70 expression in three kinds of shell colors Japanese scallop Mizuhopecten yessoensis under high temperature stress |
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| Abstract: |
| Three shell colors of Mizuhopecten yessoensis were used to test the survival rate, oxygen consumption rate, ammonia-N excretion rate, catalase (CAT) and activity of superoxide dismutase (SOD) activities, total-antioxidant capacity (T-AOC), peroxidase (POD) contents, and HSP70 under short-term high temperature. The scallops reared in 15℃ were acclimatized to the different temperature levels 20, 22, 24 and 26℃ (temperature increased 1℃ per day), and then maintained for 7 days. The results showed that the survival rates of the 3 shell colors M. yessoensis showed a increase among 15～24℃ temperature treatments with the increase of temperature, and then decrease followed. The survival rates of white shell colors M. yessoensis were higher than the two other shell colors in each temperature treatment. The oxygen consumption rates and ammonia-N excretion rates in 3 shell colors M. yessoensis exhibited the similar changes with the survival rates. In addition, four kinds of immune enzyme activity (T- AOC, SOD, CAT and POD) also showed a similar changes with the survival rates( a firstly increase, and then decrease followed). And these 4 kinds of immune enzyme activity in white shell colors M. yessoensis were higher than the two other shell colors in each temperature treatment. The HSP70 expression in three shell colors M. yessoensis continued to express when the temperature increased, and the highest value occurred in 26℃. The white shell colors scallop showed the higher relative quantity of HSP 70 expression (5.07) compared with the other two shell colors scallops. Finally, we get the conclusion that white shell colors scallop has a strong ability of resistance to high temperature, and it could be a very good alternative materials in breeding M. yessoensis new strains with strong high temperature resistant in the future. |
| Key words: Mizuhopecten yessoensis shell color high temperature immune indicators HSP70 |
