| 摘要: |
| 为对大菱鲆(Scophthalmus maximus)的养殖群体进行微卫星分析研究, 作者用70个微卫星位点在大菱鲆亲本后代中的基因型分离方式进行检测与分析。结果显示, 70个位点共产生了12种分离方式(♀×♂)。70个微卫星位点中45个位点符合孟德尔遗传定律(P≥0.05), 有25个位点偏离了孟德尔遗传定律(P<0.05)。平均等位基因数目为2.2, 平均有效等位基因数为1.885。平均期望杂合度和观测杂合度分别为0.55和0.537。多态信息含量为0.182～0.699, 平均值为0.361。研究证明这些标记大部分可以用于大菱鲆进一步的图谱构建, QTL定位和分子标记辅助育种研究。同时对群体的分析表明, 该大菱鲆养殖群体遗传多样性水平较高, 可用于进一步的良种繁育。 |
| 关键词: 大菱鲆(Scophthalmus maximus) 微卫星位点 遗传变异 基因分离 |
| DOI：10.11759//hykx20141028002 |
| 分类号: |
| 基金项目:国家863 计划(2012AA10A408-8); 现代农业产业技术体系建设专项资金共同资助(CARS-50-01) |
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| Separation and analysis of turbot microsatellite markers in progeny |
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WANG Guang-ning,MA Ai-jun,LI Meng,MA De-you
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| Abstract: |
| To evaluate the farmed turbot population using microsatellite markers, we analyzed the segregation patterns of genotypes constituted by 70 microsatellite loci in turbot parents and their progeny. Results revealed that a total of 12 patterns were detected through sexual hybridization (♀×♂). A total of 45 loci were found to adhere to the Mendel’s law of inheritance (P≥0.05), whereas the remaining 25 deviated from the law (P<0.05). The average number of alleles and effective number of alleles were 2.2 and 1.885, respectively. The average expected and observed heterozygosities were 0.55 and 0.537, respectively. The range of polymorphic information content was between 0.182 and 0.699, with an average of 0.361. These results demonstrate that most of these markers will be useful for future progress in genetic breeding in turbot and the farmed turbot population with a high level of genetic diversity can be used for the further breeding of improved varieties. |
| Key words: turbot microsatellite loci genetic variation gene isolation |
